製品の概要

  • 製品名Anti-RASGRP 4 antibody
    RASGRP 4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to RASGRP 4
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: CGLCCHKHCR DQVKVECKKR PGAKGDAGPP GAPVPSTPAP HASCGSEENH SYTLSLEPET GCQLRHAWTQ TESPHPSWET DTVPCPVMDP PSTASSKLDS , corresponding to amino acids 574-674 of Human RASGRP 4

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.
    • 組織特異性Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient.
    • 配列類似性Belongs to the RASGRP family.
      Contains 1 EF-hand domain.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 Ras-GEF domain.
    • 発生段階Expressed early during development. Detected in all fetal tissues tested.
    • ドメインThe phorbol-ester/DAG-type zinc finger mediates the binding and the functional activation by DAG.
    • 細胞内局在Cytoplasm. Cell membrane. Recruited to membranes upon activation by DAG.
    • Information by UniProt
    • 参照データベース
    • 別名
      • GRP4_HUMAN antibody
      • Guanyl nucleotide releasing protein 4 antibody
      • RAS guanyl releasing protein 4 antibody
      • RAS guanyl-releasing protein 4 antibody
      • RASGRP4 antibody
      see all

    Anti-RASGRP 4 antibody 画像

    • RASGRP 4 antibody (ab57978) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
    • Western blot against tagged recombinant protein immunogen using ab57978 RASGRP 4 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-RASGRP 4 antibody (ab57978) 使用論文

    ab57978 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab57978.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"