製品の概要

  • 製品名Anti-RASA1 antibody [EP536Y]
    RASA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP536Y] to RASA1
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GAP aa 250-350 (internal sequence). From near the SH3 domain.
    Database link: P20936

  • ポジティブ・コントロール
    • Rat brain lysate, human ovary carcinoma tissue, HeLa cells.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab40677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ICC
  • 追加情報ICC: 1/250 - 1/500.
    IHC-P: 1/50.

    WB: 1/2000. Detects a band of approximately 140 kDa (predicted molecular weight: 96 kDa).

    Is unsuitable for FACS or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.
    • 組織特異性In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).
    • 関連疾患Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
      Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
      Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.
    • 配列類似性Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 Ras-GAP domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • 翻訳後修飾The N-terminus is blocked.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CM AVM antibody
      • CMAVM antibody
      • DKFZp434N071 antibody
      • GAP antibody
      • GTPase activating protein antibody
      • GTPase-activating protein antibody
      • OTTHUMP00000222390 antibody
      • OTTHUMP00000222391 antibody
      • OTTHUMP00000222392 antibody
      • OTTHUMP00000222393 antibody
      • p120GAP antibody
      • p120RASGAP antibody
      • PKWS antibody
      • Ras GTPase-activating protein 1 antibody
      • RAS p21 protein activator (GTPase activating protein) 1 antibody
      • Ras p21 protein activator antibody
      • RASA antibody
      • RASA1 antibody
      • RASA1_HUMAN antibody
      • RasGAP antibody
      • Triphosphatase activating protein antibody
      see all

    Anti-RASA1 antibody [EP536Y] 画像

    • Anti-RASA1 antibody [EP536Y] (ab40677) at 1/2000 dilution + Rat brain lysate at 10 µg

      Predicted band size : 96 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible cleavage fragment).
    • Immunohistochemical analysis of paraffin embedded human ovary carcinoma using ab40677 diluted 1:50.Immunohistochemical analysis of paraffin embedded human ovary carcinoma using ab40677 diluted 1:50.
    • Immunofluorescent staining of HeLa cells using ab40677 diluted 1:250.

    Anti-RASA1 antibody [EP536Y] (ab40677) 使用論文

    This product has been referenced in:
    • Du C  et al. Hypoxia-inducible MiR-182 promotes angiogenesis by targeting RASA1 in hepatocellular carcinoma. J Exp Clin Cancer Res 34:67 (2015). WB, IHC ; Human . Read more (PubMed: 26126858) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry.

    I can confirm the ICC testing protocol for which I have copied below. I hope this will be helpful. Please note, this would be a guideline only and may require some individual optimization.

    With regards ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"