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ab134640 Ras GTPase ELISA Kit is designed specifically for the study of Ras activation and can be used to study novel signaling pathways for activating Ras. The kit can also be used as a diagnostic test to detect oncogenic Ras related to malignancy. Ras GTPase ELISA Kits contain a Raf-RBD protein fused to GST that will be coated onto the provided 96-well (12 x 8 well strips), glutathione-coated plate. The activated Ras contained in cellular extract specifically binds to Raf-RBD, while inactive Ras does not bind. Bound Ras is detected by incubating with a primary antibody that detects H-Ras in mouse and H- & K-Ras in human samples. Addition of a secondary antibody conjugated to horseradish peroxidase (HRP) and developing solution provides a sensitive chemiluminescent readout that is easily quantified by luminescence. The 96-well plate with individual strips of 12 wells is suitable for manual use or high-throughput screening applications.
Ras GTPase ELISA Kit specifically detects activated H- and K-Ras in Human and H-Ras in rodent samples.
|内容||1 x 96 tests|
|10X Antibody Binding Buffer||1 x 2.2ml|
|10X Wash Buffer||2 x 22ml|
|96-well assay plate||1 unit|
|Anti-rat HRP-conjugated IgG||1 x 11µl|
|Chemiluminescent Reagent||1 x 2ml|
|GST-Raf-RBD||4 x 25µl|
|HeLa whole-cell extract (EGF treated)||2 x 40µl|
|H-Ras antibody||1 x 11µl|
|Lysis Buffer||1 x 50ml|
|Plate sealer||1 unit|
|Protease Inhibitor Cocktail||1 x 500µl|
|Reaction Buffer||1 x 4ml|
Our Abpromise guarantee covers the use of ab134640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
Quantification of activated Ras: Increasing amounts of whole-cell extracts from unstimulated 293T/17 and EGF stimulated HeLa cells were assayed for Ras activity using ab134640 Ras GTPase ELISA Kit. To illustrate the Kit’s specificity for activated Ras, 293T/17 cells which do not contain basal levels of activated Ras were used as a negative control. Data was also shown for unstimulated HeLa cells, which do contain basal levels of activated Ras. This data is provided for demonstration only.
ab134640 has not yet been referenced specifically in any publications.