製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Raptor
  • 特異性
    This antibody reacts with Raptor. Raptor has multiple isoforms that may also be recognized by this antibody.
  • アプリケーション
    適用あり: ICC/IF, WB, ICCmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A 15 amino acid synthetic peptide from the central region of human Raptor

  • ポジティブ・コントロール
    • L1210 cell lysate and L1210 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
ICC Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
  • 組織特異性
    Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
  • 配列類似性
    Belongs to the WD repeat RAPTOR family.
    Contains 7 WD repeats.
  • 細胞内局在
    Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA1303 antibody
    • KOG1 antibody
    • Mip1 antibody
    • P150 target of rapamycin (TOR) scaffold protein antibody
    • p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody
    • P150 target of rapamycin (TOR)-scaffold protein antibody
    • Raptor antibody
    • Regulatory associated protein of mTOR antibody
    • Regulatory associated protein of MTOR complex 1 antibody
    • Regulatory-associated protein of mTOR antibody
    • RPTOR antibody
    • RPTOR_HUMAN antibody
    see all

画像

  • Lane 1 : Anti-Raptor antibody (ab62454) at 2 µg/ml
    Lane 2 : Anti-Raptor antibody (ab62454) at 4 µg/ml

    Lane 1 : L1210 cell lysate
    Lane 2 : L1210 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 149 kDa
    Observed band size : 149 kDa
  • Immunocytochemical staining of L1210 cells using Raptor antibody at 1µg/ml.
  • Immunofluorescence of Raptor in L1210 cells using ab62454 at 10 ug/ml.

参考文献

ab62454 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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