Anti-Raptor 抗体 (ab62454)
Key features and details
- Rabbit polyclonal to Raptor
- Suitable for: WB, ICC/IF, ICC
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Raptor antibody
Raptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Raptor -
由来種
Rabbit -
特異性
This antibody reacts with Raptor. Raptor has multiple isoforms that may also be recognized by this antibody. -
アプリケーション
適用あり: WB, ICC/IF, ICCmore details -
種交差性
交差種: Mouse -
免疫原
A 15 amino acid synthetic peptide from the central region of human Raptor
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ポジティブ・コントロール
- L1210 cell lysate and L1210 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62454の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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ICC |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
ICC
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. -
組織特異性
Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. -
配列類似性
Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats. -
細胞内局在
Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability. - Information by UniProt
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参照データベース
- Entrez Gene: 74370 Mouse
- SwissProt: Q8K4Q0 Mouse
- Unigene: 209933 Mouse
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別名
- KIAA1303 antibody
- KOG1 antibody
- Mip1 antibody
see all
画像
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Lane 1 : Anti-Raptor antibody (ab62454) at 2 µg/ml
Lane 2 : Anti-Raptor antibody (ab62454) at 4 µg/ml
All lanes : L1210 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 149 kDa
Observed band size: 149 kDa -
Immunocytochemical staining of L1210 cells using Raptor antibody at 1µg/ml.
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Immunofluorescence of Raptor in L1210 cells using ab62454 at 10 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab62454 は論文での使用が確認できていません。