Anti-Raptor 抗体 (ab125351)
Key features and details
- Rabbit polyclonal to Raptor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Raptor antibody
Raptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Raptor -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human Raptor aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab155813) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following human whole cell lysates: HepG2; HeLa; HEK293; PANC1; A431.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125351の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/250. Detects a band of approximately 151 kDa (predicted molecular weight: 149 kDa).
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特記事項 |
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WB
1/250. Detects a band of approximately 151 kDa (predicted molecular weight: 149 kDa). |
ターゲット情報
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機能
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. -
組織特異性
Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. -
配列類似性
Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats. -
細胞内局在
Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability. - Information by UniProt
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参照データベース
- Entrez Gene: 57521 Human
- Omim: 607130 Human
- SwissProt: Q8N122 Human
- Unigene: 133044 Human
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別名
- KIAA1303 antibody
- KOG1 antibody
- Mip1 antibody
see all
画像
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All lanes : Anti-Raptor antibody (ab125351) at 1/250 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 149 kDa
Observed band size: 151 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125351 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab125351 は論文での使用が確認できていません。