製品の概要

  • 製品名Anti-RAP80 antibody
    RAP80 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RAP80
  • アプリケーション適用あり: IP, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 600 and 650 of human RAP80 (NP_057374.3).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
WB
  • 追加情報IP: Use at 2-5µg/mg of lysate.
    WB: 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
    • 組織特異性Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
    • 配列類似性Belongs to the RAP80 family.
      Contains 2 UIM (ubiquitin-interacting motif) repeats.
    • ドメインThe UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
      The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
    • 翻訳後修飾Sumoylated.
      Phosphorylated upon DNA damage by ATM or ATR.
    • 細胞内局在Nucleus. Localizes at sites of DNA damage at double-strand breaks.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BRCA1-A complex subunit RAP80 antibody
      • Nuclear zinc finger protein RAP80 antibody
      • OTTHUMP00000161441 antibody
      • OTTHUMP00000223372 antibody
      • OTTHUMP00000223374 antibody
      • RAP80 antibody
      • Receptor associated protein 80 antibody
      • Receptor-associated protein 80 antibody
      • Retinoid X receptor interacting protein 110 antibody
      • Retinoid x receptor interacting protein antibody
      • Retinoid X receptor-interacting protein 110 antibody
      • RIP110 antibody
      • Rxrip110 antibody
      • Ubiquitin interaction motif containing 1 antibody
      • Ubiquitin interaction motif containing protein 1 antibody
      • Ubiquitin interaction motif-containing protein 1 antibody
      • UIMC1 antibody
      • UIMC1_HUMAN antibody
      • X2HRIP110 antibody
      see all

    Anti-RAP80 antibody 画像

    • All lanes : Anti-RAP80 antibody (ab70822) at 1 µg/ml

      Lane 1 : HeLa whole cell lysate at 15 µg
      Lane 2 : HeLa whole cell lysate at 5 µg
      Lane 3 : 293T whole cell lysate at 50 µg


      Predicted band size : 80 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 300 kDa,65 kDa,80 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human RAP80 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70822 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

    Anti-RAP80 antibody (ab70822) 使用論文

    ab70822 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (MCF7 cells)
    Specification MCF7 cells
    Fixative Methanol
    Permeabilization Yes - Acetone
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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    投稿 Jul 21 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"