製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RAP80
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 650 and the C-terminus (residue 719) of Human RAP80 (NP_057374.3).

  • ポジティブ・コントロール
    • Human Breast Carcinoma, Ovarian Carcinoma and Testicular Seminoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab114855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

ターゲット情報

  • 機能
    Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
  • 組織特異性
    Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
  • 配列類似性
    Belongs to the RAP80 family.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • ドメイン
    The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions.
    The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas.
  • 翻訳後修飾
    Sumoylated.
    Phosphorylated upon DNA damage by ATM or ATR.
  • 細胞内局在
    Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BRCA1-A complex subunit RAP80 antibody
    • Nuclear zinc finger protein RAP80 antibody
    • OTTHUMP00000161441 antibody
    • OTTHUMP00000223372 antibody
    • OTTHUMP00000223374 antibody
    • RAP80 antibody
    • Receptor associated protein 80 antibody
    • Receptor-associated protein 80 antibody
    • Retinoid X receptor interacting protein 110 antibody
    • Retinoid x receptor interacting protein antibody
    • Retinoid X receptor-interacting protein 110 antibody
    • RIP110 antibody
    • Rxrip110 antibody
    • Ubiquitin interaction motif containing 1 antibody
    • Ubiquitin interaction motif containing protein 1 antibody
    • Ubiquitin interaction motif-containing protein 1 antibody
    • UIMC1 antibody
    • UIMC1_HUMAN antibody
    • X2HRIP110 antibody
    see all

画像

  • ab114855, at 1/250 dilution, staining RAP80 in formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry with DAB staining.
  • ab114855, at 1/250 dilution, staining RAP80 in formalin-fixed, paraffin-embedded Human seminoma tissue by Immunohistochemistry with DAB staining.
  • ab114855, at 1/100 dilution, staining RAP80 in formalin-fixed, paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed antibody Hilyte PlusTM 555 at 1/100 dilution.

プロトコール

参考文献

ab114855 has not yet been referenced specifically in any publications.

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