製品の概要

  • 製品名Anti-RAP2B antibody
    RAP2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RAP2B
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow, Zebrafish
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 159 of Human RAP2B (AAH12362)

  • ポジティブ・コントロール
    • A431 whole cell lysate. H1299 and HepG2 cell lines. CL1-0 Xenograft tissue.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab101369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer pH 8.0 is recommended

ターゲット情報

  • 機能Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells.
  • 組織特異性Expressed in red blood cells (at protein level).
  • 配列類似性Belongs to the small GTPase superfamily. Ras family.
  • ドメインThe effector domain mediates the interaction with RUNDC3A.
  • 翻訳後修飾Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.
  • 細胞内局在Recycling endosome membrane. Associated with red blood cells-released vesicles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC20484 antibody
    • RAP 2B antibody
    • RAP2A antibody
    • Rap2b antibody
    • RAP2B member of RAS oncogene family antibody
    • RAP2B_HUMAN antibody
    • Ras family small GTP binding protein RAP2B antibody
    • Ras related protein RAP 2B antibody
    • Ras related protein RAP2B antibody
    • Ras-related protein Rap-2b antibody
    • Small GTP binding protein antibody
    see all

Anti-RAP2B antibody 画像

  • Anti-RAP2B antibody (ab101369) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 21 kDa
  • Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using ab101369 at 1/100 dilution.

Anti-RAP2B antibody (ab101369) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

The freezing point of a 20% glycerol solution is about -5C, so freezing at -20C is expected. If you have aliquotted the antibody into small volumes for single experiments, then this will not be a problem. Freezing and thawing can be damaging but not al...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"