製品の概要

  • 製品名Anti-RAP1GAP antibody [Y135]
    RAP1GAP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y135] to RAP1GAP
  • アプリケーション適用あり: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the C terminus of human Rap1GAP.

  • ポジティブ・コントロール
    • WB: Jurkat cell lysate. IHC-P: Human stomach carcinoma.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 73 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

ターゲット情報

  • 機能GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
  • 組織特異性Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
  • 配列類似性Contains 1 GoLoco domain.
    Contains 1 Rap-GAP domain.
  • 細胞内局在Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0474 antibody
    • Rap1 GTPase activating protein 1 antibody
    • RAP1 GTPase activating protein antibody
    • Rap1 GTPase-activating protein 1 antibody
    • RAP1GA1 antibody
    • Rap1ga1 protein antibody
    • Rap1GAP antibody
    • Rap1GAP1 antibody
    • RAP1GAPII antibody
    • RAPGAP antibody
    • RPGP1_HUMAN antibody
    see all

Anti-RAP1GAP antibody [Y135] 画像

  • Anti-RAP1GAP antibody [Y135] (ab32119) at 1/10000 dilution + Jurkat cell lysate

    Predicted band size : 73 kDa
    Observed band size : 85-95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Ab32119, at a dilution of 1/100, staining RAP1GA1 in paraffin embedded human stomach carcinoma tissue by immunohistochemistry.

Anti-RAP1GAP antibody [Y135] (ab32119) 使用論文

ab32119 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"