Anti-RAP1GAP 抗体 [Y135] (ab32119)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y135] to RAP1GAP
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RAP1GAP antibody [Y135]
RAP1GAP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y135] to RAP1GAP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human RAP1GAP aa 600-700 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Jurkat cell lysate. IHC-P: Human stomach carcinoma.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y135 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32119の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 73 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/5000 - 1/10000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 73 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state. -
組織特異性
Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues. -
配列類似性
Contains 1 GoLoco domain.
Contains 1 Rap-GAP domain. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5909 Human
- Entrez Gene: 110351 Mouse
- Entrez Gene: 313644 Rat
- Omim: 600278 Human
- SwissProt: P47736 Human
- SwissProt: A2ALS5 Mouse
- Unigene: 148178 Human
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別名
- KIAA0474 antibody
- Rap1 GTPase activating protein 1 antibody
- RAP1 GTPase activating protein antibody
see all
画像
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Anti-RAP1GAP antibody [Y135] (ab32119) at 1/10000 dilution + Jurkat cell lysate
Predicted band size: 73 kDa
Observed band size: 85-95 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
Ab32119, at a dilution of 1/100, staining RAP1GA1 in paraffin embedded human stomach carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab32119 は 5 報の論文で使用されています。
- Cao JZ et al. UBE2C promotes the progression of pancreatic cancer and glycolytic activity via EGFR stabilization-mediated PI3K-Akt pathway activation. J Gastrointest Oncol 13:1444-1453 (2022). PubMed: 35837197
- Wang Y et al. The degradation of Rap1GAP via E6AP-mediated ubiquitin-proteasome pathway is associated with HPV16/18-infection in cervical cancer cells. Infect Agent Cancer 16:71 (2021). PubMed: 34952616
- Deng QF et al. Cyclooxygenase-2 mediates gefitinib resistance in non-small cell lung cancer through the EGFR/PI3K/AKT axis. J Cancer 11:3667-3674 (2020). PubMed: 32284763
- Hu DD et al. SKA3 promotes lung adenocarcinoma metastasis through the EGFR-PI3K-Akt axis. Biosci Rep 40:N/A (2020). PubMed: 32068236
- Xie B et al. PIAS1 protects against myocardial ischemia-reperfusion injury by stimulating PPAR? SUMOylation. BMC Cell Biol 19:24 (2018). PubMed: 30419807