Anti-RAP1A + RAP1B 抗体 [EPR14815(B)] (ab181858)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14815(B)] to RAP1A + RAP1B
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-RAP1A + RAP1B antibody [EPR14815(B)]
RAP1A + RAP1B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14815(B)] to RAP1A + RAP1B -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- HeLa, A431, 293 and MOLT4 cell lysates; HeLa cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR14815(B) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181858の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP |
1/50.
|
|
WB |
1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
特記事項 |
---|
Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/50. |
WB
1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
ターゲット情報
-
関連性
RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known. -
細胞内局在
Cell Membrane; Attached to the membrane by a lipid anchor -
参照データベース
- Entrez Gene: 5906 Human
- Entrez Gene: 5908 Human
- Entrez Gene: 109905 Mouse
- Entrez Gene: 215449 Mouse
- Entrez Gene: 171337 Rat
- Entrez Gene: 295347 Rat
- Omim: 179520 Human
- Omim: RAP1B Human
see all -
別名
- C21KG antibody
- G 22K antibody
- GTP binding protein smg p21A antibody
see all
画像
-
All lanes : Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858) at 1/50000 dilution
Lane 1 : Hela cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MOLT4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling RAP1B with ab181858 at a 1/30 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
-
Western blot analysis on immunoprecipitation from 1) 293 cell lysate or 2) negative control, labeling RAP1B using ab181858 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (1)
ab181858 は 1 報の論文で使用されています。
- Shen Y & Ding W Therapeutic Hypothermia Mitigates the Sepsis-Increased Permeability in EA. hy926 Cells by Preserving Rap1 Expression. Ther Hypothermia Temp Manag 11:201-207 (2021). PubMed: 33709787