製品の概要

  • 製品名Anti-RAP1A + RAP1B antibody [EP879Y]
    RAP1A + RAP1B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP879Y] to RAP1A + RAP1B
  • アプリケーション適用あり: WB, IP, ICCmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N terminus of human RAP1A + RAP1B

  • ポジティブ・コントロール
    • Human brain lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP 1/40.
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    • 関連性RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known.
    • 細胞内局在Cell Membrane; Attached to the membrane by a lipid anchor
    • 参照データベース
    • 別名
      • C21KG antibody
      • G 22K antibody
      • GTP binding protein smg p21A antibody
      • GTP binding protein smg p21B antibody
      • K REV antibody
      • KREV 1 antibody
      • KREV antibody
      • KREV1 antibody
      • RAL 1B antibody
      • RAL1B antibody
      • RAP 1 antibody
      • RAP 1A antibody
      • RAP 1B antibody
      • RAP1 antibody
      • RAP1A member of RAS oncogene family antibody
      • RAP1B antibody
      • RAP1B member of RAS oncogene family antibody
      • Ras family small GTP binding protein RAP1B antibody
      • RAS related protein 1b antibody
      • Ras related protein Krev 1 antibody
      • Ras related protein Krev1 antibody
      • Ras related protein RAP 1A antibody
      • Ras related protein RAP 1B antibody
      • RAS related protein RAP1A antibody
      • Ras related protein RAP1B antibody
      • small GTP binding protein antibody
      • SMGP 21 antibody
      • SMGP21 antibody
      see all

    Anti-RAP1A + RAP1B antibody [EP879Y] 画像

    • Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/500 dilution + human brain lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 21 kDa
      Observed band size : 21 kDa

      Secondary antibody - goat anti-rabbit HRP (ab6721)

    • Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/1000 dilution + Mouse brain

      Predicted band size : 21 kDa

      Secondary antibody - goat anti-rabbit HRP (ab6721)

    Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) 使用論文

    This product has been referenced in:
    • Todorovic V  et al. Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation. Mol Biol Cell 25:3749-64 (2014). Read more (PubMed: 25208567) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HEPG2)
    Specification HEPG2
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Fixative Paraformaldehyde
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    投稿 Nov 18 2015

    Application Western blot
    Sample Human Cell lysate - whole cell (HEPG2)
    Gel Running Conditions Reduced Denaturing (12.5)
    Loading amount 20 µg
    Specification HEPG2
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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    投稿 Nov 13 2015

    Application Western blot
    Loading amount 100000 cells
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
    Specification mouse embryonic stem cells
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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    Verified customer

    投稿 Jan 15 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"