Anti-RAP1/TERF2IP 抗体 [4c8/1] (ab14404)
Key features and details
- Mouse monoclonal [4c8/1] to RAP1/TERF2IP
- Suitable for: IP, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-RAP1/TERF2IP antibody [4c8/1]
RAP1/TERF2IP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4c8/1] to RAP1/TERF2IP -
由来種
Mouse -
アプリケーション
適用あり: IP, WB, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human RAP1/TERF2IP.
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ポジティブ・コントロール
- In Western Blot, this antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; HepG2; A431.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
4c8/1 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14404の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use a concentration of 5 µg/ml.
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
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Flow Cyt |
特記事項 |
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IP
Use a concentration of 5 µg/ml. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
ターゲット情報
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機能
Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes. -
組織特異性
Ubiquitous. Highly expressed. -
配列類似性
Belongs to the RAP1 family.
Contains 1 BRCT domain.
Contains 1 Myb-like domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm. Chromosome. Chromosome > telomere. Associates with chromosomes, both at telomeres and in extratelomeric sites. Also exists as a cytoplasmic form, where it associates with the IKK complex. - Information by UniProt
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参照データベース
- Entrez Gene: 54386 Human
- Omim: 605061 Human
- SwissProt: Q9NYB0 Human
- Unigene: 301419 Human
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別名
- Dopamine receptor interacting protein 5 antibody
- Dopamine receptor-interacting protein 5 antibody
- DRIP 5 antibody
see all
画像
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All lanes : Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes -
RAP1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to RAP1/TERF2IP RITM0035008 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab14404.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 55kDa; RAP1 -
Overlay histogram showing HeLa cells stained with ab14404 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14404, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab14404 は 6 報の論文で使用されています。
- Xu Q et al. Vascular senescence in progeria: role of endothelial dysfunction. Eur Heart J Open 2:oeac047 (2022). PubMed: 36117952
- Pennarun G et al. Increase in lamin B1 promotes telomere instability by disrupting the shelterin complex in human cells. Nucleic Acids Res 49:9886-9905 (2021). PubMed: 34469544
- Zhang B et al. Arecoline Enhances Phosphodiesterase 4A Activity to Promote Transforming Growth Factor-β-Induced Buccal Mucosal Fibroblast Activation via cAMP-Epac1 Signaling Pathway. Front Pharmacol 12:722040 (2021). PubMed: 34819854
- Pereira CD et al. Nuclear Accumulation of LAP1:TRF2 Complex during DNA Damage Response Uncovers a Novel Role for LAP1. Cells 9:N/A (2020). PubMed: 32751253
- Liu H et al. NLRP3 inflammasome induced liver graft injury through activation of telomere-independent RAP1/KC axis. J Pathol 242:284-296 (2017). PubMed: 28378341
- Wang J et al. Vascular Smooth Muscle Cell Senescence Promotes Atherosclerosis and Features of Plaque Vulnerability. Circulation 132:1909-19 (2015). PubMed: 26416809