製品の概要
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製品名Anti-RANTES antibody
RANTES 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to RANTES
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由来種Rabbit
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アプリケーション適用あり: Indirect ELISA, ELISA, WBmore details
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種交差性交差種: Rat
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免疫原
Highly pure (>98%) recombinant rRANTES (rat RANTES)
製品の特性
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製品の状態Lyophilised:Reconstitute with 200µl of sterile water.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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バッファーPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプunknown
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軽鎖の種類unknown
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab9783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Indirect ELISA | ||
| ELISA | ||
| WB |
追加情報
ELISA:To detect rRANTES by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rRANTES.
Western Blot:To detect rRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rRANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot:To detect rRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rRANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ターゲット情報
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機能Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
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組織特異性T-cell and macrophage specific.
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配列類似性Belongs to the intercrine beta (chemokine CC) family.
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翻訳後修飾N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity. -
細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 81780 Rat
- SwissProt: P50231 Rat
- Unigene: 8019 Rat
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別名
- Beta chemokine RANTES antibody
- Beta chemokine RANTES precursor antibody
- C C motif chemokine 5 antibody
see all
画像
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Indirect ELISA - Anti-RANTES antibody (ab9783)Image from Tripathy D & Grammas P,J Neuroinflammation. 2009 Mar 16;6:10. Fig 5.; doi:10.1186/1742-2094-6-10; 16 March 2009, Journal of Neuroinflammation 2009, 6:10Indirect ELISA measuring RANTES levels using ab9783.
Supernatants collected from rat cerebral cortical cells after various treatments were coated onto 96-well flat bottom plates and incubated overnight at 4°C. The plates were blocked with 1% BSA at 37°C for 45 min before incubation with primary antibody (1/1000) in bicarbonate buffer overnight at 4°C. An HRP-conjugated secondary IgG (1/1000) was added and incubated for 45 min at 37°C, in the dark. The reaction was developed by adding o-phenylene diamine H2O2 for 20 min. Optical density was measured at 450 nm.
プロトコール
参考文献
This product has been referenced in:
- Zhang Y et al. Pretreatment with a combination of ligustrazine and berberine improves cardiac function in rats with coronary microembolization. Acta Pharmacol Sin 37:463-72 (2016). WB ; Rat . Read more (PubMed: 26924290) »
- Tripathy D & Grammas P Acetaminophen protects brain endothelial cells against oxidative stress. Microvasc Res 77:289-96 (2009). ELISA ; Rat . Read more (PubMed: 19265712) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"