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製品の概要

  • 製品名
    Anti-RANTES antibody
    RANTES 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RANTES
  • アプリケーション
    適用あり: Indirect ELISA, ELISA, WBmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Highly pure (>98%) recombinant rRANTES (rat RANTES)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Indirect ELISA
ELISA
WB
  • 追加情報
    ELISA:To detect rRANTES by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rRANTES.

    Western Blot:To detect rRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rRANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    • ターゲット情報

    • 機能
      Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
    • 組織特異性
      T-cell and macrophage specific.
    • 配列類似性
      Belongs to the intercrine beta (chemokine CC) family.
    • 翻訳後修飾
      N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
      The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
      • 別名
        • Beta chemokine RANTES antibody
        • Beta chemokine RANTES precursor antibody
        • C C motif chemokine 5 antibody
        • CCL 5 antibody
        • CCL5 antibody
        • CCL5_HUMAN antibody
        • Chemokine (C C motif) ligand 5 antibody
        • Chemokine CC Motif Ligand 5 antibody
        • D17S136E antibody
        • EoCP antibody
        • Eosinophil chemotactic cytokine antibody
        • MGC17164 antibody
        • RANTES(4-68) antibody
        • Regulated upon activation normally T expressed and presumably secreted antibody
        • SCYA 5 antibody
        • SCYA5 antibody
        • SIS delta antibody
        • SIS-delta antibody
        • SISd antibody
        • Small inducible cytokine A5 (RANTES) antibody
        • Small inducible cytokine A5 antibody
        • Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
        • Small-inducible cytokine A5 antibody
        • T cell specific protein p288 antibody
        • T cell specific RANTES protein antibody
        • T cell-specific protein P228 antibody
        • T-cell-specific protein RANTES antibody
        • TCP 228 antibody
        • TCP228 antibody
        see all

      Anti-RANTES antibody 画像

      • Indirect ELISA - Anti-RANTES antibody (ab9783)
        Indirect ELISA - Anti-RANTES antibody (ab9783)Image from Tripathy D & Grammas P,J Neuroinflammation. 2009 Mar 16;6:10. Fig 5.; doi:10.1186/1742-2094-6-10; 16 March 2009, Journal of Neuroinflammation 2009, 6:10
        Indirect ELISA measuring RANTES levels using ab9783.

        Supernatants collected from rat cerebral cortical cells after various treatments were coated onto 96-well flat bottom plates and incubated overnight at 4°C. The plates were blocked with 1% BSA at 37°C for 45 min before incubation with primary antibody (1/1000) in bicarbonate buffer overnight at 4°C. An HRP-conjugated secondary IgG (1/1000) was added and incubated for 45 min at 37°C, in the dark. The reaction was developed by adding o-phenylene diamine H2O2 for 20 min. Optical density was measured at 450 nm.

      プロトコール

      Anti-RANTES antibody (ab9783) 使用論文

      This product has been referenced in:
      • Zhang Y  et al. Pretreatment with a combination of ligustrazine and berberine improves cardiac function in rats with coronary microembolization. Acta Pharmacol Sin 37:463-72 (2016). WB ; Rat . Read more (PubMed: 26924290) »
      • Tripathy D & Grammas P Acetaminophen protects brain endothelial cells against oxidative stress. Microvasc Res 77:289-96 (2009). ELISA ; Rat . Read more (PubMed: 19265712) »

      See all 5 Publications for this product

      ab9783 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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