製品の概要

  • 製品名Anti-RANTES antibody
    RANTES 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to RANTES
  • アプリケーション適用あり: ICC/IF, IHC-Fr, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use a concentration of 0.5 - 5 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 8 kDa.
ELISA Use a concentration of 0.5 - 1 µg/ml.

ターゲット情報

  • 機能Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
  • 組織特異性T-cell and macrophage specific.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻訳後修飾N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
    The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta chemokine RANTES antibody
    • Beta chemokine RANTES precursor antibody
    • C C motif chemokine 5 antibody
    • CCL 5 antibody
    • CCL5 antibody
    • CCL5_HUMAN antibody
    • Chemokine (C C motif) ligand 5 antibody
    • Chemokine CC Motif Ligand 5 antibody
    • D17S136E antibody
    • EoCP antibody
    • Eosinophil chemotactic cytokine antibody
    • MGC17164 antibody
    • RANTES(4-68) antibody
    • Regulated upon activation normally T expressed and presumably secreted antibody
    • SCYA 5 antibody
    • SCYA5 antibody
    • SIS delta antibody
    • SIS-delta antibody
    • SISd antibody
    • Small inducible cytokine A5 (RANTES) antibody
    • Small inducible cytokine A5 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
    • Small-inducible cytokine A5 antibody
    • T cell specific protein p288 antibody
    • T cell specific RANTES protein antibody
    • T cell-specific protein P228 antibody
    • T-cell-specific protein RANTES antibody
    • TCP 228 antibody
    • TCP228 antibody
    see all

Anti-RANTES antibody 画像

  • ab10590 staining RANTES in human endothelial cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using Triton, blocked with 1% BSA for 1 hour at 25°C and then incubated with ab10590 at 2µg/ml for 1 hour at 25°C. The secondary used was an FITC conjugated goat anti-rabbit polyclonal used at a 1/100 dilution. Counter stained with TO-PRO3.

    See Abreview

  • ab10590 staining RANTES in Human liver cancer metastases by Immunohistochemistry (Frozen sections).

    Tissue was fixed with paraformaldehyde, blocked with 5% serum for 1 hour at 24°C and permeabilized with Triton X-100. Samples were incubated with primary antibody (in PBS + 0.3% Triton X-100 + 1% BSA) for 3 hours at 24°C. An AlexaFluor®488-conjugated donkey anti-goat IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-RANTES antibody (ab10590) 使用論文

This product has been referenced in:
  • Muthuswamy R  et al. NF-?B hyperactivation in tumor tissues allows tumor-selective reprogramming of the chemokine microenvironment to enhance the recruitment of cytolytic T effector cells. Cancer Res 72:3735-43 (2012). IHC-Fr ; Human . Read more (PubMed: 22593190) »

See 1 Publication for this product

Product Wall

The best choice from what we have is the goat polyclonal ab10590. Other antibodies we have that are raised against RANTES may also work for immunocytochemistry but they have not been tested in this application.

The exception is the rabbit mo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Liver mets)
Specification Liver mets
Fixative Paraformaldehyde
Permeabilization Yes - Triton-x-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ravikumar Muthuswamy

Verified customer

投稿 Jul 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Recombinant protein)
Loading amount 0.1 µg
Specification Recombinant protein
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Endothelial cell)
Specification Endothelial cell
Fixative Paraformaldehyde
Permeabilization Yes - Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"