Anti-RANKL 抗体 (ab65024)
Key features and details
- Rabbit polyclonal to RANKL
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RANKL antibody
RANKL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RANKL -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide derived from an internal sequence of human RANKL
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65024の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/500 - 1/1000.
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WB |
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
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特記事項 |
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ICC/IF
1/500 - 1/1000. |
WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa). |
ターゲット情報
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機能
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
組織特異性
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
関連疾患
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
細胞内局在
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8600 Human
- Entrez Gene: 21943 Mouse
- Omim: 602642 Human
- SwissProt: O14788 Human
- SwissProt: O35235 Mouse
- Unigene: 333791 Human
- Unigene: 249221 Mouse
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別名
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
see all
画像
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ab65024 at 1/500 - 1/1000 dilution staining RANKL in HepG2 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
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All lanes : Anti-RANKL antibody (ab65024) at 1/500 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with immunising peptide
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab65024 は 7 報の論文で使用されています。
- Li X et al. QiangGuYin Modulates the OPG/RANKL/RANK Pathway by Increasing Secretin Levels during Treatment of Primary Type I Osteoporosis. Evid Based Complement Alternat Med 2021:7114139 (2021). PubMed: 34754319
- Shen Y et al. The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction. Mol Med Rep 21:2113-2122 (2020). PubMed: 32323761
- Yeo L et al. Expression of FcRL4 defines a pro-inflammatory, RANKL-producing B cell subset in rheumatoid arthritis. Ann Rheum Dis N/A:N/A (2014). Human . PubMed: 24431391
- Laury AM et al. Periostin and receptor activator of nuclear factor ?-B ligand expression in allergic fungal rhinosinusitis. Int Forum Allergy Rhinol 4:716-24 (2014). PubMed: 25060295
- Holt V et al. Identification of a subpopulation of marrow MSC-derived medullary adipocytes that express osteoclast-regulating molecules: marrow adipocytes express osteoclast mediators. PLoS One 9:e108920 (2014). IF ; Human . PubMed: 25302610
- Vijayan V et al. Homocysteine alters the osteoprotegerin/RANKL system in the osteoblast to promote bone loss: pivotal role of the redox regulator forkhead O1. Free Radic Biol Med 61C:72-84 (2013). PubMed: 23500899
- Yeo L et al. Cytokine mRNA profiling identifies B cells as a major source of RANKL in rheumatoid arthritis. Ann Rheum Dis : (2011). IHC-Fr ; Human . PubMed: 21742639