製品の概要

  • 製品名Anti-RanGAP1 antibody [1B4]
    RanGAP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1B4] to RanGAP1
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Rat, Dog, Human
  • 免疫原

    Recombinant full length Human RanGAP1 produced in HEK293T cells (NP_002874).

  • ポジティブ・コントロール
    • HEK293T cell lysate transfected with pCMV6-ENTRY RanGAP1 cDNA; HEK293T cells transfected with RanGAP1 overexpress plasmid; Human liver, prostate and Human ovary adenocarcinoma tissues; HeLa and Jurkat cells; HepG2, HeLa, Jurkat, HT29, A549, MDCK, PC12 and MCF7 cell extracts.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 63 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

ターゲット情報

  • 機能GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
  • 組織特異性Highly expressed in brain, thymus and testis.
  • 配列類似性Belongs to the RNA1 family.
    Contains 6 LRR (leucine-rich) repeats.
  • 翻訳後修飾Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
    Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
  • 細胞内局在Cytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Fug 1 antibody
    • Fug1 antibody
    • GTPase-activating protein, RAN, 1 antibody
    • KIAA1835 antibody
    • MGC20266 antibody
    • OTTHUMP00000028918 antibody
    • OTTHUMP00000198755 antibody
    • OTTHUMP00000198756 antibody
    • OTTHUMP00000198757 antibody
    • OTTHUMP00000198758 antibody
    • RAGP1_HUMAN antibody
    • Ran 1 antibody
    • RAN GTPase activating protein 1 antibody
    • Ran GTPase-activating protein 1 antibody
    • Ran1 antibody
    • RANGAP 1 antibody
    • RANGAP antibody
    • RanGAP1 antibody
    • SD antibody
    • Segregation distorter homolog antibody
    • Segregation distortion antibody
    see all

Anti-RanGAP1 antibody [1B4] 画像

  • All lanes : Anti-RanGAP1 antibody [1B4] (ab119092) at 1/1000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY RanGAP1 cDNA

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique

    Predicted band size : 63 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202683)
  • All lanes : Anti-RanGAP1 antibody [1B4] (ab119092) at 1/1000 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 63 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202667)
  • ab119092 at 1/50 dilution staining RanGAP1 in paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • ab119092 at 1/50 dilution staining RanGAP1 in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab119092 at 1/50 dilution staining RanGAP1 in paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • ab119092 at 1/100 dilution staining RanGAP1 in COS7 cells transiently transfected with pCMV6-ENTRY RanGAP1, by Immunofluorescence.
  • ab119092 at 1/100 dilution staining RanGAP1 in HEK293T cells transfected with either pCMV6-ENTRY RanGAP1 overexpress plasmid (Red) or empty vector control plasmid (Blue) and analysed by Flow Cytometry.
  • ab119092 at 1/100 dilution staining RanGAP1 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab119092 at 1/100 dilution staining RanGAP1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

Anti-RanGAP1 antibody [1B4] (ab119092) 使用論文

ab119092 has not yet been referenced specifically in any publications.

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