製品の概要

  • 製品名Anti-RANBP6 antibody
    RANBP6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RANBP6
  • 特異性ab74448 detects endogenous levels of total RANBP6 protein.
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human RANBP6.

  • ポジティブ・コントロール
    • K562, COLO and COS7 cell extracts. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney. IF/ICC: A549 cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab74448 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 125 kDa.
ELISA 1/10000.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性RANBP6 (Ran-binding protein 6) belongs to the importin beta family and may function in nuclear protein import as a nuclear transport receptor.
  • 細胞内局在Cytoplasmic and Nuclear
  • 参照データベース
  • 別名
    • C630001B19 antibody
    • FLJ11120 antibody
    • FLJ54311 antibody
    • OTTHUMP00000021039 antibody
    • OTTHUMP00000021040 antibody
    • RAN binding protein 6 antibody
    • Ran GTP binding protein antibody
    • RANBP 6 antibody
    see all

Anti-RANBP6 antibody 画像

  • ICC/IF image of ab74448 stained A549 cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74448, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • All lanes : Anti-RANBP6 antibody (ab74448) at 1/500 dilution

    Lane 1 : K562 cell extracts
    Lane 2 : COLO cell extracts
    Lane 3 : COS7 cell extracts
    Lane 4 : COS7 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 125 kDa
    Observed band size : 125 kDa
  • IHC image of RANBP6 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74448, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

Anti-RANBP6 antibody (ab74448) 使用論文

ab74448 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"