Anti-RanBP2 抗体 [mAbcam58385] (ab58385)

製品の概要

  • 製品名Anti-RanBP2 antibody [mAbcam58385]
    RanBP2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam58385] to RanBP2
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 3100 - 3200 of Human RanBP2.

    (Peptide available as ab59186.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human whole cell lysates: Hela, HEK293, Ramos. This antibody gave a positive result in IHC in the following FFPE tissue: Human colon adenocarcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 360 kDa (predicted molecular weight: 360 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1.
  • パスウェイProtein modification; protein sumoylation.
  • 関連疾患Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem.
  • 配列類似性Contains 1 PPIase cyclophilin-type domain.
    Contains 4 RanBD1 domains.
    Contains 8 RanBP2-type zinc fingers.
    Contains 1 TPR repeat.
  • ドメインContains F-X-F-G repeats.
  • 翻訳後修飾Polyubiquitinated by PARK2, which leads to proteasomal degradation.
  • 細胞内局在Nucleus > nuclear pore complex. Cytoplasmic filaments.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 358 kDa nucleoporin antibody
    • ANE1 antibody
    • E3 SUMO-protein ligase RanBP2 antibody
    • IIAE3 antibody
    • Nuclear pore complex protein Nup358 antibody
    • Nucleoporin 358 antibody
    • Nucleoporin Nup358 antibody
    • NUP358 antibody
    • p270 antibody
    • RAN binding protein 2 antibody
    • Ran-binding protein 2 antibody
    • RANBP2 antibody
    • RBP2_HUMAN antibody
    • Transformation related protein 2 antibody
    • TRP 1 antibody
    • TRP 2 antibody
    • TRP1 antibody
    • TRP2 antibody
    see all

Anti-RanBP2 antibody [mAbcam58385] 画像

  • IHC image of RanBP2 staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58385, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-RanBP2 antibody [mAbcam58385] (ab58385) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 360 kDa
    Observed band size : 360 kDa
    Additional bands at : 220 kDa,230 kDa. We are unsure as to the identity of these extra bands.
  • Overlay histogram showing HeLa cells stained with ab58385 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58385, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-RanBP2 antibody [mAbcam58385] (ab58385) 使用論文

ab58385 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Jul 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"