Anti-RanBP2 抗体 (ab112061)
Key features and details
- Rabbit polyclonal to RanBP2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-RanBP2 antibody
RanBP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RanBP2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 1050-1100 of Human RanBP2 (NP_006258.3).
-
ポジティブ・コントロール
- HeLa Whole cell lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab112061 was affinity purified using an epitope specific to RanBP2 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab112061の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 358 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 358 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
機能
E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates. Could also have isomerase or chaperone activity and may bind RNA or DNA. Component of the nuclear export pathway. Specific docking site for the nuclear export factor exportin-1. -
パスウェイ
Protein modification; protein sumoylation. -
関連疾患
Defects in RANBP2 are the cause of susceptibility to encephalopathy acute necrotizing type 1 (ANE1) [MIM:608033]. A rapidly progressive encephalopathy manifesting in susceptibile individuals with seizures and coma. It can occur within days in otherwise healthy children after common viral infections such as influenza and parainfluenza, without evidence of viral infection of the brain or inflammatory cell infiltration. Brain T2-weighted magnetic resonance imaging reveals characteristic symmetric lesions present in the thalami, pons and brainstem. -
配列類似性
Contains 1 PPIase cyclophilin-type domain.
Contains 4 RanBD1 domains.
Contains 8 RanBP2-type zinc fingers.
Contains 1 TPR repeat. -
ドメイン
Contains F-X-F-G repeats. -
翻訳後修飾
Polyubiquitinated by PARK2, which leads to proteasomal degradation. -
細胞内局在
Nucleus > nuclear pore complex. Cytoplasmic filaments. - Information by UniProt
-
参照データベース
- Entrez Gene: 5903 Human
- Omim: 601181 Human
- SwissProt: P49792 Human
- Unigene: 199561 Human
- Unigene: 715056 Human
-
別名
- 358 kDa nucleoporin antibody
- ANE1 antibody
- E3 SUMO-protein ligase RanBP2 antibody
see all
画像
-
All lanes : Anti-RanBP2 antibody (ab112061) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 358 kDa
Exposure time: 10 seconds -
Detection of RanBP2 by Western Blot of Immunprecipitate.
ab112061 at 0.1µg/ml staining RanBP2 in HeLa whole cell lysate immunoprecipitated using ab112061 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (2)
ab112061 は 2 報の論文で使用されています。
- Yatskevich S et al. Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation. Cell Rep 34:108929 (2021). PubMed: 33789095
- Zhu T et al. Neddylation controls basal MKK7 kinase activity in breast cancer cells. Oncogene 35:2624-33 (2016). PubMed: 26364603