製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to RALY
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length human RALY (AAI03754.1).

  • ポジティブ・コントロール
    • HepG2 cell lysate; RALY transfected 293T cell lysate and HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing.
  • 組織特異性
    Expressed in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Weakly expressed in placenta.
  • 配列類似性
    Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Autoantigen p542 antibody
    • Heterogeneous nuclear ribonucleoprotein C-like 2 antibody
    • hnRNP associated with lethal yellow protein homolog antibody
    • hnRNP core protein C-like 2 antibody
    • MGC117312 antibody
    • P542 antibody
    • RALY antibody
    • RALY_HUMAN antibody
    • RNA binding protein (autoantigenic hnRNP associated with lethal yellow) antibody
    • RNA binding protein (autoantigenic) antibody
    • RNA binding protein autoantigenic (hnRNP associated with lethal yellow homolog (mouse)) antibody
    • RNA-binding protein Raly antibody
    see all

画像

  • Anti-RALY antibody (ab88616) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • All lanes : Anti-RALY antibody (ab88616) at 1 µg/ml

    Lane 1 : RALY transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab88616 at 10µg/ml staining RALY in HeLa cells by Immunofluorescence.

参考文献

ab88616 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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