製品の概要

  • 製品名Anti-RALY antibody
    RALY 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RALY
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • 免疫原

    A region within synthetic peptide: QENTTSEAGL PQGEARTRDD GDEEGLLTHS EEELEHSQDT DADDGALQ, corresponding to C terminal amino acids 243-290 of Human RALY

  • ポジティブ・コントロール
    • Jurkat cell lysate. Human muscle tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab66263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing.
  • 組織特異性Expressed in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Weakly expressed in placenta.
  • 配列類似性Belongs to the RRM HNRPC family. RALY subfamily.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Autoantigen p542 antibody
    • Heterogeneous nuclear ribonucleoprotein C-like 2 antibody
    • hnRNP associated with lethal yellow protein homolog antibody
    • hnRNP core protein C-like 2 antibody
    • MGC117312 antibody
    • P542 antibody
    • RALY antibody
    • RALY_HUMAN antibody
    • RNA binding protein (autoantigenic hnRNP associated with lethal yellow) antibody
    • RNA binding protein (autoantigenic) antibody
    • RNA binding protein autoantigenic (hnRNP associated with lethal yellow homolog (mouse)) antibody
    • RNA-binding protein Raly antibody
    see all

Anti-RALY antibody 画像

  • Anti-RALY antibody (ab66263) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labeling RALY with ab66263 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

Anti-RALY antibody (ab66263) 使用論文

This product has been referenced in:

See 1 Publication for this product

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