製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RALA
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 1-179 of Human RALA (AAH39858).

  • ポジティブ・コントロール
    • HepG2 whole cell lysate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.

ターゲット情報

  • 機能
    Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells.
  • 配列類似性
    Belongs to the small GTPase superfamily. Ras family.
  • 翻訳後修飾
    Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs).
  • 細胞内局在
    Cell surface. Cell membrane. Cleavage furrow. Midbody. Prior to LPA treatment found predominantly at the cell surface and in the presence of LPA co-localizes with LPAR1 and LPAR2 in the endocytic vesicles. During early cytokinesis localizes at the cleavage furrow membrane. Colocalizes with EXOC2 at the early midbody ring and persists there till maturation of the midbody.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC48949 antibody
    • Ral a antibody
    • Ral A protein antibody
    • RAL antibody
    • RALA antibody
    • RALA_HUMAN antibody
    • Ras family small GTP binding protein RALA antibody
    • RAS like protein A antibody
    • Ras related protein RalA antibody
    • Ras-related protein Ral-A antibody
    • v ral simian leukemia viral oncogene homolog A (ras related) antibody
    • v ral simian leukemia viral oncogene homolog A antibody
    see all

画像

  • Anti-RALA antibody (ab96759) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 24 kDa
  • Anti-RALA antibody (ab96759) at 1/3000 dilution + Recombinant Human RALA protein (ab102555) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

プロトコール

参考文献

ab96759 has not yet been referenced specifically in any publications.

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