リコンビナント
RabMAb

Anti-RAIDD 抗体 [EPR1640Y] (ab52621)

製品の概要

  • 製品名
    Anti-RAIDD antibody [EPR1640Y]
    RAIDD 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1640Y] to RAIDD
  • アプリケーション
    適用あり: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues from the C terminus of human RAIDD.

  • ポジティブ・コントロール
    • HeLa cell lysate and Human heart muscle tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex.
  • 組織特異性
    Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
  • 配列類似性
    Contains 1 CARD domain.
    Contains 1 death domain.
  • ドメイン
    Contains a death domain involved in the binding of RIP protein.
    The CARD domain mediates the interaction with caspase-2.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CASP2 and RIPK1 domain containing adaptor with death domain antibody
    • Caspase and RIP adapter with death domain antibody
    • Caspase and RIP adaptor with death domain antibody
    • Cradd antibody
    • CRADD_HUMAN antibody
    • Death adaptor molecule RAIDD antibody
    • Death domain containing protein CRADD antibody
    • Death domain-containing protein CRADD antibody
    • MGC9163 antibody
    • RIP associated ICH1/CED3 homologous protein with death domain antibody
    • RIP associated protein with a death domain antibody
    • RIP-associated protein with a death domain antibody
    see all

画像

  • Anti-RAIDD antibody [EPR1640Y] (ab52621) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • ab52621, at a 1/50 dilution, staining Human RAIDD in heart muscle, using Immunohistochemistry, Paraffin embedded tissue.

参考文献

This product has been referenced in:
  • Di Donato N  et al. Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant. Am J Hum Genet 99:1117-1129 (2016). Read more (PubMed: 27773430) »
  • Shen Q  et al. Regulation of CRADD-caspase 2 cascade by histone deacetylase 1 in gastric cancer. Am J Transl Res 6:538-47 (2014). Read more (PubMed: 25360218) »

See all 3 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab52621.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録