製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RAI1
  • アプリケーション
    適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to N terminal residues of Human RAI1

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution. PubMed: 20738874
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 203 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 関連性
    RAI1 (retinoid-acid induced protein 1) may be involved in neuronal differentiation. RAI1 is highly similar to its mouse counterpart and is expressed at high levels mainly in neuronal tissues. RAI1 has a polymorphic polyglutamine tract in it's N-terminal domain. Expression of the mouse counterpart in neurons is induced by retinoic acid. The RAI1 gene is associated with both the severity of the phenotype and the response to medication in schizophrenic patients. Defects in RAI1 are a cause of Smith-Magenis syndrome (SMS). There are four named isoforms.
  • 細胞内局在
    Cytoplasmic and Nuclear. In neurons it is localized to neurites.
  • 参照データベース
  • 別名
    • DKFZP434A139 antibody
    • KIAA1820 antibody
    • MGC12824 antibody
    • retinoic acid induced 1 antibody
    • Retinoid acid induced protein 1 antibody
    • SMCR antibody
    • SMS antibody
    see all

画像

  • ab58658 staining RAI1 (green) in murine N2a cells transfected with RAI1-HA wild type and mutant forms, by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200). An AlexaFluor®488-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

参考文献

This product has been referenced in:
  • Fragoso YD  et al. Expression in the human brain of retinoic acid induced 1, a protein associated with neurobehavioural disorders. Brain Struct Funct 220:1195-203 (2015). WB, IHC-P ; Human . Read more (PubMed: 24519454) »
  • Carmona-Mora P  et al. Functional and cellular characterization of human Retinoic Acid Induced 1 (RAI1) mutations associated with Smith-Magenis Syndrome. BMC Mol Biol 11:63 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20738874) »

See all 2 Publications for this product

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