製品の概要

  • 製品名Anti-RAGE antibody
    RAGE 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to RAGE
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Dog, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 42-59 of Human RAGE.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution. PubMed: 16941014See Abreviews. Suggested working dilution of 1/400 from PMID 16941014.

ターゲット情報

  • 機能Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • 組織特異性Endothelial cells.
  • 配列類似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Advanced glycosylation end product-specific receptor antibody
    • Ager antibody
    • MGC2235 antibody
    • RAGE_HUMAN antibody
    • Receptor for advanced glycosylation end products antibody
    see all

Anti-RAGE antibody 画像

  • ab7764 at 1/100 staining mouse skin tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was blocked in serum and then incubated with the antibody. A Cy2 conjugated donkey anti-goat (F(ab')2 fragment) was used as the secondary antibody.

    See Abreview

  • ab7764 staining RAGE in mouse lung tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before antigen retrieval which involved boiling citric acid and then blocking at 370C for 15 minutes was performed. The primary antibody was diluted in 1/250 and it was incubated with sample for 1 hour at 37°C. A HRP conjugated rat polyclonal to goat IgG was used undiluted as a secondary antibody.

    See Abreview

Anti-RAGE antibody (ab7764) 使用論文

This product has been referenced in:
  • Sterenczak KA  et al. Quantitative PCR and immunohistochemical analyses of HMGB1 and RAGE expression in canine disseminated histiocytic sarcoma (malignant histiocytosis). Anticancer Res 31:1541-8 (2011). IHC-P ; Dog . Read more (PubMed: 21617208) »
  • Morbini P  et al. The receptor for advanced glycation end products and its ligands: a new inflammatory pathway in lung disease? Mod Pathol 19:1437-45 (2006). Read more (PubMed: 16941014) »

See all 2 Publications for this product

Product Wall

Thank you for your phone call. I'd be happy to issue you either a credit or refund. Alternatively, a free of charge replacement with another antibody can be sent. I have checked our catalog for other potential suitable RAGE antibodies to ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Other
Permeabilization No
Blocking step (Other company) kit as blocking agent as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Jun 12 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (brain)
Specification brain
Fixative formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Vlady Pavlova

Verified customer

投稿 Jan 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Carol Artlett

Verified customer

投稿 Dec 24 2007

Thank you for your enquiry. Ab7764 is supplied in an unpurified form, whole antiserum, and we are unable to provide an exact concentration for unpurified material. However, this wholeserum is expected to have a concentration of around 0.5-1mg/ml of ant...

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Unfortunately, we are not aware of any publications that feature the use of this antibody.

This antibody has been shown to work in IHC on formalin fixed paraffin embedded (FFPE) tissues . Antibodies which work on FFPE tissues usually work on frozen tissues. However, being as it has not been specifically tested, we can't guarantee results

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To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product.

Cross-reactivity to mouse or rat has not yet been tested. The antibody was raised against the peptide sequence 42-59aa, although we do not know the sequnce homology with rat and mouse. As this antibody is a polyclonal there are several epitopes along t...

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This antibody has been used at 1:250,000 in ELISA when using the immunizing peptide. However, since this was with the synthetic peptide we would of course expect high specificity, so we would suggest the standard working concentration of between 1-5u...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"