Anti-RAGE 抗体 (ab37647)
Key features and details
- Rabbit polyclonal to RAGE
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RAGE antibody
RAGE 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RAGE -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Synthetic peptide:
KGAPKKPPQRLEWKLNTGRTC
, corresponding to amino acids 39-58 of Human RAGE -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab37647の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (4) |
Use a concentration of 1 µg/ml.
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WB | (6) |
1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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Flow Cyt | (1) |
Use 1µg for 106 cells.
Use PBS/EDTA to detach cells to preserve the glycoproteins on the cell surface; do not fix, do not permeabilise.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
Flow Cyt
Use 1µg for 106 cells. Use PBS/EDTA to detach cells to preserve the glycoproteins on the cell surface; do not fix, do not permeabilise.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. -
組織特異性
Endothelial cells. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 280986 Cow
- Entrez Gene: 177 Human
- Entrez Gene: 11596 Mouse
- Entrez Gene: 81722 Rat
- Omim: 600214 Human
- SwissProt: Q28173 Cow
- SwissProt: Q15109 Human
- SwissProt: Q62151 Mouse
see all -
別名
- Advanced glycosylation end product-specific receptor antibody
- Ager antibody
- DAMA 358M23.4 antibody
see all
画像
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All lanes : Anti-RAGE antibody (ab37647) at 1/500 dilution
Lane 1 : HUVEC whole cell lysate
Lane 2 : EVC304 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 17,40 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
The band at 52kDa is a tubulin loading control. -
RAW 264.7 cells were stained with anti-RAGE (1ug/million cells) for 1hr at 4 deg C followed by staining with anti-rabbit IgG-PE conjugate. Cells were analyzed by flow cytometry. Unstained cells or cells stained with secondary antibody alone are represented in the background
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ab37647 at a 1/100 dilution staining RAGE in bovine endothelial cells by Immunocytochemistry/ Immunofluorescence. Fixed in PFA, permeabilized with Triton X-100. Blocked using 10% serum for 20 minutes at room temperature. Secondary used at 1/200 polyclonal Goat snti-rabbit conjugated to Alexa Fluor 568 (red). Nuclear staining (blue).
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All lanes : Anti-RAGE antibody (ab37647) at 1/2000 dilution
Lane 1 : Bovine lung extract
Lane 2 : Mouse lung extract
Lane 3 : HeLa cells expressing human soluble RAGE
Secondary
All lanes : anti-rabbit IgG alkaline phosphatase conjugate
Predicted band size: 42 kDa
Observed band size: 42 kDa
Bovine RAGE is slightly larger than human and mouse RAGE - 44kDa compared with 42kDa -
ab37647 staining human Jurkat T cells by Flow Cytometry. The cells were prepared in PBS with 0.2% BSA. The primary antibody diluted 1/100 and incubated with sample for 30 minutes at 0°C. The secondary antibody was Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200.
Specimen tube 006 is negative control
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ICC/IF image of ab37647 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37647, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (70)
ab37647 は 70 報の論文で使用されています。
- Peng X et al. RAGE mediates airway inflammation via the HDAC1 pathway in a toluene diisocyanate-induced murine asthma model. BMC Pulm Med 22:61 (2022). PubMed: 35148729
- Nowicka N et al. The Involvement of RAGE and Its Ligands during Progression of ALS in SOD1 G93A Transgenic Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35216298
- Xia B et al. Lycopene Improves Bone Quality and Regulates AGE/RAGE/NF-кB Signaling Pathway in High-Fat Diet-Induced Obese Mice. Oxid Med Cell Longev 2022:3697067 (2022). PubMed: 35222796
- Selejan SR et al. Renal denervation reduces atrial remodeling in hypertensive rats with metabolic syndrome. Basic Res Cardiol 117:36 (2022). PubMed: 35834066
- Olson LC et al. Human Adipose-Derived Stromal Cells Delivered on Decellularized Muscle Improve Muscle Regeneration and Regulate RAGE and P38 MAPK. Bioengineering (Basel) 9:N/A (2022). PubMed: 36134970