製品の概要

  • 製品名Anti-Raf1 (phospho S43) antibody
    Raf1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Raf1 (phospho S43)
  • 特異性Detects enogenous levels of Raf1 only when phosphorylated at serine 43
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic phosphopeptide from around the phosphorylation site of serine 43 (RASPDD) of human Raf1

  • ポジティブ・コントロール
    • NIH/3T3 cell extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ELISA 1/5000.

ターゲット情報

  • 機能Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
  • 組織特異性In skeletal muscle, isoform 1 is more abundant than isoform 2.
  • 関連疾患Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
    Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
  • 細胞内局在Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c Raf antibody
    • C-raf antibody
    • C-Raf proto-oncogene, serine/threonine kinase antibody
    • CMD1NN antibody
    • Craf 1 transforming gene antibody
    • cRaf antibody
    • Craf1 transforming gene antibody
    • EC 2.7.11.1 antibody
    • kinase Raf1 antibody
    • Murine sarcoma 3611 oncogene 1 antibody
    • NS5 antibody
    • Oncogene MIL antibody
    • Oncogene RAF1 antibody
    • OTTHUMP00000160218 antibody
    • OTTHUMP00000207813 antibody
    • OTTHUMP00000209389 antibody
    • Protein kinase raf 1 antibody
    • Proto-oncogene c-RAF antibody
    • Raf 1 antibody
    • Raf 1 proto oncogene serine/threonine kinase antibody
    • RAF antibody
    • Raf proto oncogene serine/threonine protein kinase antibody
    • RAF proto-oncogene serine/threonine-protein kinase antibody
    • RAF-1 antibody
    • RAF1 antibody
    • RAF1_HUMAN antibody
    • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
    • TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody
    • v raf 1 murine leukemia viral oncogene homolog 1 antibody
    • v-raf murine sarcoma viral oncogene homolog 1 antibody
    • vraf1 murine leukemia viral oncogene homolog 1 antibody
    see all

Anti-Raf1 (phospho S43) antibody 画像

  • All lanes : Anti-Raf1 (phospho S43) antibody (ab61027) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with immunising phosphopeptide


    Predicted band size : 73 kDa
    Observed band size : 73 kDa

Anti-Raf1 (phospho S43) antibody (ab61027) 使用論文

This product has been referenced in:
  • Liu D  et al. Sulfur dioxide inhibits vascular smooth muscle cell proliferation via suppressing the Erk/MAP kinase pathway mediated by cAMP/PKA signaling. Cell Death Dis 5:e1251 (2014). WB ; Rat . Read more (PubMed: 24853429) »

See 1 Publication for this product

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