製品の概要

  • 製品名Anti-Raf1 antibody [RNP1]
    Raf1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [RNP1] to Raf1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Xenopus laevis, Orangutan
  • 免疫原

    Synthetic peptide:

    GQRDSSYYWEIE

    , corresponding to internal sequence amino acids 334-345 of Human Raf1

  • ポジティブ・コントロール
    • Extracts and cells from A431 cell line (Human Epidermoid carcinoma)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 73 kDa.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 機能Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
  • 組織特異性In skeletal muscle, isoform 1 is more abundant than isoform 2.
  • 関連疾患Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
    Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity.
  • 細胞内局在Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c Raf antibody
    • C-raf antibody
    • C-Raf proto-oncogene, serine/threonine kinase antibody
    • CMD1NN antibody
    • Craf 1 transforming gene antibody
    • cRaf antibody
    • Craf1 transforming gene antibody
    • EC 2.7.11.1 antibody
    • kinase Raf1 antibody
    • Murine sarcoma 3611 oncogene 1 antibody
    • NS5 antibody
    • Oncogene MIL antibody
    • Oncogene RAF1 antibody
    • OTTHUMP00000160218 antibody
    • OTTHUMP00000207813 antibody
    • OTTHUMP00000209389 antibody
    • Protein kinase raf 1 antibody
    • Proto-oncogene c-RAF antibody
    • Raf 1 antibody
    • Raf 1 proto oncogene serine/threonine kinase antibody
    • RAF antibody
    • Raf proto oncogene serine/threonine protein kinase antibody
    • RAF proto-oncogene serine/threonine-protein kinase antibody
    • RAF-1 antibody
    • RAF1 antibody
    • RAF1_HUMAN antibody
    • Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
    • TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody
    • v raf 1 murine leukemia viral oncogene homolog 1 antibody
    • v-raf murine sarcoma viral oncogene homolog 1 antibody
    • vraf1 murine leukemia viral oncogene homolog 1 antibody
    see all

Anti-Raf1 antibody [RNP1] 画像

  • Lane 1 : Without Raf1 antibody [RNP1] (ab50858)
    Lane 2 : Anti-Raf1 antibody [RNP1] (ab50858) at 1 µg/ml

    Lane 1 : A431cell extract (Human Epidermoid carcinoma)
    Lane 2 : A431cell extract (Human Epidermoid carcinoma)

    Secondary
    Goat Anti-Mouse, Peroxidase conjugate

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
  • Localisation of RAF1 protein in the cytoplasm of A431. A431 cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. The cells were incubated with Monoclonal Anti-RAF1 (ab50858) and further developed with Rabbit Anti-Mouse IgG, FITC-conjugate.

Anti-Raf1 antibody [RNP1] (ab50858) 使用論文

ab50858 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab50858.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"