リコンビナント
RabMAb

Anti-Radixin 抗体 [EP1862Y] (ab52495)

製品の概要

  • 製品名
    Anti-Radixin antibody [EP1862Y]
    Radixin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1862Y] to Radixin
  • アプリケーション
    適用あり: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Radixin aa 350-450 (N terminal).

  • ポジティブ・コントロール
    • WB: HeLa cell lysate IHC-P: Paraffin embedded human ovarian carcinoma
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IP 1/100.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250.

ターゲット情報

  • 機能
    Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
  • 関連疾患
    Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 配列類似性
    Contains 1 FERM domain.
  • ドメイン
    The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.
  • 翻訳後修飾
    Phosphorylated by tyrosine-protein kinases.
  • 細胞内局在
    Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CB567 antibody
    • CG12537 antibody
    • DFNB24 antibody
    • ESP10 antibody
    • Hh-induced MATH and BTB domain-containing protein antibody
    • HIB antibody
    • Moesin-B antibody
    • Protein roadkill antibody
    • RADI_HUMAN antibody
    • Radixin antibody
    • RDX antibody
    see all

画像

  • Anti-Radixin antibody [EP1862Y] (ab52495) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 80 kDa
    Observed band size : 80 kDa
  • Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using ab52495 at 1/100 dilution.
  • ICC/IF image of ab52495 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52495, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab52495 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52495, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Wu KX  et al. Human genome-wide RNAi screen reveals host factors required for enterovirus 71 replication. Nat Commun 7:13150 (2016). Read more (PubMed: 27748395) »
  • Ji X  et al. Retinal Pigment Epithelium Atrophy 1 (rpea1): A New Mouse Model With Retinal Detachment Caused by a Disruption of Protein Kinase C, ?. Invest Ophthalmol Vis Sci 57:877-88 (2016). Read more (PubMed: 26978024) »

See all 10 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
30 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Mar 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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