Anti-Radixin 抗体 [AF1H1] (ab85323)

製品の概要

  • 製品名
    Anti-Radixin antibody [AF1H1]
    Radixin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AF1H1] to Radixin
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    His tagged recombinant fragment, corresponding to amino acids 1-310 of Human Radixin, from E.coli.

  • ポジティブ・コントロール
    • A431, HeLa, HepG2, K562, 293T, PC12 and NIH3T3 cell lysates.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.03% Sodium Azide
    Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • ポリ/モノ
    モノクローナル
  • クローン名
    AF1H1
  • アイソタイプ
    IgG2b
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能
    Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
  • 関連疾患
    Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 配列類似性
    Contains 1 FERM domain.
  • ドメイン
    The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ablilty to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN.
  • 翻訳後修飾
    Phosphorylated by tyrosine-protein kinases.
  • 細胞内局在
    Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CB567 antibody
    • CG12537 antibody
    • DFNB24 antibody
    • ESP10 antibody
    • Hh-induced MATH and BTB domain-containing protein antibody
    • HIB antibody
    • Moesin-B antibody
    • Protein roadkill antibody
    • RADI_HUMAN antibody
    • Radixin antibody
    • RDX antibody
    see all

Anti-Radixin antibody [AF1H1] 画像

  • All lanes : Anti-Radixin antibody [AF1H1] (ab85323) at 1/10000 dilution

    Lane 1 : A431 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : HepG2 cell lysates
    Lane 4 : K562 cell lysates
    Lane 5 : 293T cell lysates
    Lane 6 : PC12 cell lysates
    Lane 7 : NIH3T3 cell lysates


    Predicted band size : 69 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-Radixin antibody [AF1H1] (ab85323) 使用論文

ab85323 has not yet been referenced specifically in any publications.

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I apologise for the delay, I contacted my lab colleagues to check whether this antibody has ever been tested in IP applications. They confirmed it was once used in the past with bad results, therefore we wouldn&rs...

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Thank you for contacting us.

Unfortunately, the exact concentration of the Anti-Radixin antibody [AF1H1] cannot be determined.

This monoclonal antibody isproduced by ammonium sulfate precipitation from ascites. Different lots of thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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