Anti-Rad9 抗体 - ChIP Grade (ab70810)


  • 製品名Anti-Rad9 antibody - ChIP Grade
    Rad9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rad9 - ChIP Grade
  • アプリケーション適用あり: ChIP/Chip, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residue 350 and the C-terminus (residue 391) of Human Rad9 (NP_004575.1)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and 293T cells.




Our Abpromise guarantee covers the use of ab70810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP/Chip Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
IP Use at 2-5 µg/mg of lysate.


  • 機能Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • 配列類似性Belongs to the rad9 family.
  • 翻訳後修飾Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell cycle checkpoint control protein antibody
    • Cell cycle checkpoint control protein RAD9A antibody
    • DNA repair exonuclease rad9 homolog A antibody
    • hRAD 9 antibody
    • hRAD9 antibody
    • Rad 9 antibody
    • RAD 9A antibody
    • RAD9 (S pombe) homolog antibody
    • RAD9 homolog A antibody
    • RAD9 homolog antibody
    • Rad9a antibody
    • RAD9A_HUMAN antibody
    see all

Anti-Rad9 antibody - ChIP Grade 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Rad9 with ab70810 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Rad9 antibody - ChIP Grade (ab70810) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 43 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Exposure time : 3 minutes
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated using ab70810 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70810 at 1ug/ml.
    Detection: chemiluminescence with exposure time of 30 seconds.
  • IHC image of ab70810 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Rad9 antibody - ChIP Grade (ab70810) 使用論文

ab70810 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP on chip
Sample Human Cell lysate - nuclear (prostate cancer cells)
Specification prostate cancer cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Microarray
Positive control RAD9 has been comfirmed to bind p21 promoter region, so after I precipatated DNA with RAD9 abs, I used precipiated DNA as template, with specific primers for RT-PCR and check p21 promoter region. AB70810 gave positive results as previous reported.

Yes, Following qPCR, I did CHIP microarray with annealing CHIP'd DNA. I got excited data, and now I am doing the following research.

other company RAD9 antibodies, INPUT
Negative control mouse IgG

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投稿 Jul 31 2012

AB70810 DISCOUNT CODE: XXXX Expiration date: AB117848 DISCOUNT CODE: XXXX Expiration date: I am very pleased to hear you would like to accept our offer and test both ab70810 and ab117848 and in ChIP. Above are the code that I have created for each...

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Thank you for contacting us. While we do not have a ChIP graded RAD9A antibody. It is highly likely that the products we do have may work in your experiment. Ideally, specific antibodies for ChIP should be affinity-purified. Polyclonal antibodies are...

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