製品の概要

  • 製品名Anti-Rad9 antibody
    Rad9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rad9
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 197 and 361 of Human Rad9 (NP_004575).

  • ポジティブ・コントロール
    • 293T, H1299, HepG2, Molt-4, Raji cell lysates. A431 and HeLa whole cell lysate.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • 配列類似性Belongs to the rad9 family.
  • 翻訳後修飾Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell cycle checkpoint control protein antibody
    • Cell cycle checkpoint control protein RAD9A antibody
    • DNA repair exonuclease rad9 homolog A antibody
    • hRAD 9 antibody
    • hRAD9 antibody
    • Rad 9 antibody
    • RAD 9A antibody
    • RAD9 (S pombe) homolog antibody
    • RAD9 homolog A antibody
    • RAD9 homolog antibody
    • Rad9a antibody
    • RAD9A_HUMAN antibody
    see all

Anti-Rad9 antibody 画像

  • All lanes : Anti-Rad9 antibody (ab96862) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 43 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96862 at 1/100 dilution.

Anti-Rad9 antibody (ab96862) 使用論文

ab96862 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab96862.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"