製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Rad9
  • アプリケーション
    適用あり: Sandwich ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Dog, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    C-QGPSPVLAEDSEGE

    , corresponding to C terminal amino acids 377-391 of Human Rad9

  • ポジティブ・コントロール
    • A431 and Daudi cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 5 - 10 µg/ml. Used as a reporter to detect recombinant RAD9A
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

  • 機能
    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • 配列類似性
    Belongs to the rad9 family.
  • 翻訳後修飾
    Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell cycle checkpoint control protein antibody
    • Cell cycle checkpoint control protein RAD9A antibody
    • DNA repair exonuclease rad9 homolog A antibody
    • hRAD 9 antibody
    • hRAD9 antibody
    • Rad 9 antibody
    • RAD 9A antibody
    • RAD9 (S pombe) homolog antibody
    • RAD9 homolog A antibody
    • RAD9 homolog antibody
    • Rad9a antibody
    • RAD9A_HUMAN antibody
    see all

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プロトコール

参考文献

ab59022 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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