The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining. Likely to work in frozen sections.
Use at an assay dependent concentration.
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
Protein modification; protein ubiquitination.
Belongs to the ubiquitin-conjugating enzyme family.
Detection of Human Rad6 by Immunohistochemistry. Sample: FFPE section of Human stomach adenocarcinoma.Antibody: ab84395 used at a dilution of 1/250. Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1/100.
Detection of Mouse Rad6 by Immunohistochemistry. Sample: FFPE section of Mouse squamous cell carcinoma.Antibody: ab84395 used at a dilution of 1/250. Detection: DAB staining using anti Rabbit IHC antibody at a dilution of 1/100.