製品の概要

  • 製品名Anti-RAD54B antibody
    RAD54B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RAD54B
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Cow, Cat
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (RRSAAPSQLQ GNSFKKPKFI PPGRSNPGLN EEITKLNPDI KLFEGVAINN) of Human RAD54B (NP_036547).

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab83311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 103 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
ICC/IF 1/200.

ターゲット情報

  • 関連性The RAD52 epistasis group genes are involved in homologous recombination, and they are conserved from yeast to humans. RAD54B is involved in DNA repair and mitotic recombination.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • DNA repair and recombination protein RAD54B antibody
    • EC 3.6.1.- antibody
    • Fibrinogen silencer binding protein antibody
    • FSBP antibody
    • RAD54 homolog B antibody
    see all

Anti-RAD54B antibody 画像

  • Anti-RAD54B antibody (ab83311) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 103 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ab83311 (1/200) staining RAD54B in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

    See Abreview

Anti-RAD54B antibody (ab83311) 使用論文

This product has been referenced in:
  • Sajesh BV  et al. Synthetic lethal targeting of superoxide dismutase 1 selectively kills RAD54B-deficient colorectal cancer cells. Genetics 195:757-67 (2013). Read more (PubMed: 24002644) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

投稿 Oct 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"