製品の概要

  • 製品名Anti-RAD54 antibody [Rad54 4E3/1]
    RAD54 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Rad54 4E3/1] to RAD54
  • アプリケーション適用あり: IP, WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名Rad54 4E3/1
  • ミエローマSp2/0-Ag14
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 84.3 kDa).
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.
  • 配列類似性Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA repair and recombination protein RAD54 like antibody
    • DNA repair and recombination protein RAD54-like antibody
    • hHR 54 antibody
    • hHR54 antibody
    • HR 54 antibody
    • HR54 antibody
    • hRAD 54 antibody
    • hRAD54 antibody
    • RAD 54A antibody
    • RAD 54L antibody
    • RAD54 (S. cerevisiae) like antibody
    • RAD54 homolog antibody
    • RAD54 like (S. cerevisiae) antibody
    • RAD54 like antibody
    • RAD54 like protein antibody
    • RAD54_HUMAN antibody
    • RAD54A antibody
    • RAD54L antibody
    see all

Anti-RAD54 antibody [Rad54 4E3/1] 画像

  • Anti-RAD54 antibody [Rad54 4E3/1] (ab11055) at 1/1000 dilution (in blocker + PBS + 0.1% Tween for 4 hours at 4°C) + HCT116p53wt cells (whole cell lysate) at 30 µg

    Secondary
    An Alexa Fluor®680 goat anti-mouse polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 84.3 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 94 kDa. We are unsure as to the identity of these extra bands.

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Overlay histogram showing HeLA cells stained with ab11055 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11055, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-RAD54 antibody [Rad54 4E3/1] (ab11055) 使用論文

This product has been referenced in:
  • Beck C  et al. PARP3 affects the relative contribution of homologous recombination and nonhomologous end-joining pathways. Nucleic Acids Res 42:5616-32 (2014). ICC/IF ; Human . Read more (PubMed: 24598253) »
  • Mason JM  et al. The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors. Cancer Res 74:3546-55 (2014). Read more (PubMed: 24753542) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 40 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells)
Total protein in input 200 µg
Specification HeLa cells
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

投稿 Apr 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116p53wt cells)
Loading amount 30 µg
Specification HCT116p53wt cells
Gel Running Conditions Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa and SK-N-SH)
Specification HeLa and SK-N-SH
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

投稿 Aug 25 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"