製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RAD52
  • 特異性
    ab61211 detects endogenous levels of total RAD52 protein.
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic non-phosphopeptide derived from human RAD52 around the phosphorylation site of tyrosine 104.

  • ポジティブ・コントロール
    • Human testis tissue and 293 cell extracts.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab61211 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab61211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

ターゲット情報

  • 機能
    Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
  • 配列類似性
    Belongs to the RAD52 family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA repair protein RAD52 antibody
    • DNA repair protein RAD52 antibody
    • DNA repair protein RAD52 homolog antibody
    • RAD 52 antibody
    • Rad52 antibody
    • RAD52 homolog (S. cerevisiae) antibody
    • RAD52 homolog (S. cerevisiae) antibody
    • RAD52 homolog antibody
    • RAD52_HUMAN antibody
    • Recombination protein RAD52 antibody
    • Recombination protein RAD52 antibody
    • Rhabdomyosarcoma antigen MU RMS 40.23 antibody
    see all

画像

  • ab61211 at 1/50 dilution staining RAD52 in human testis by Immunohistochemistry, Paraffin-embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
  • All lanes : Anti-RAD52 antibody (ab61211) at 1/500 dilution

    Lane 1 : 293 cell extracts
    Lane 2 : 293 cell extracts with immunising peptide


    Predicted band size : 46 kDa
    Observed band size : 46 kDa

参考文献

ab61211 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Mar 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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