製品の概要

  • 製品名Anti-Rad51L1 antibody
    Rad51L1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rad51L1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Dog, Chimpanzee, Macaque Monkey, Marmoset (common)
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Rad51L1.

    (Peptide available as ab99477.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; HepG2; HEK293.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

ターゲット情報

  • 機能Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.
  • 組織特異性Expressed in a wide range of tissues.
  • 関連疾患Note=A chromosomal aberration involving RAD51L1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with HMGA1.
    Note=A chromosomal aberration involving RAD51L1 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with HMGA2.
  • 配列類似性Belongs to the RecA family. RAD51 subfamily.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA repair protein RAD51 homolog 2 antibody
    • hREC2 antibody
    • MGC34245 antibody
    • OTTHUMP00000212251 antibody
    • OTTHUMP00000212253 antibody
    • OTTHUMP00000212254 antibody
    • OTTHUMP00000212255 antibody
    • R51H2 antibody
    • RA51B_HUMAN antibody
    • RAD51 homolog B (S. cerevisiae) antibody
    • RAD51 homolog B antibody
    • RAD51 like 1 antibody
    • RAD51 like protein 1 antibody
    • RAD51-like protein 1 antibody
    • Rad51B antibody
    • RAD51L1 antibody
    • REC2 antibody
    • RecA like protein antibody
    • Recombination repair protein antibody
    see all

Anti-Rad51L1 antibody 画像

  • All lanes : Anti-Rad51L1 antibody (ab87874) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Rad51L1 antibody (ab87874) 使用論文

ab87874 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

投稿 Feb 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"