製品の概要

  • 製品名Anti-Rad51 antibody
    Rad51 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rad51
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Chicken, Human
  • 免疫原

    Purified recombinant full length protein (Human)

  • ポジティブ・コントロール
    • HeLa cell extract. Normal human diploid cells.
  • 特記事項Please Click Here for Instructions For Use/Protocol

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Whole serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP Use at an assay dependent dilution.
ICC/IF 1/100.

ターゲット情報

  • 機能Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • 組織特異性Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • 関連疾患Breast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
  • 配列類似性Belongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • ドメインThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • 翻訳後修飾Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • 細胞内局在Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BRCA1/BRCA2 containing complex, subunit 5 antibody
    • BRCC 5 antibody
    • BRCC5 antibody
    • DNA repair protein RAD51 homolog 1 antibody
    • DNA repair protein rhp51 antibody
    • hRAD51 antibody
    • HsRAD51 antibody
    • HsT16930 antibody
    • MRMV2 antibody
    • Rad 51 antibody
    • RAD51 antibody
    • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
    • RAD51 homolog A antibody
    • RAD51 homolog antibody
    • RAD51 recombinase antibody
    • RAD51, S. cerevisiae, homolog of antibody
    • RAD51_HUMAN antibody
    • RAD51A antibody
    • RECA antibody
    • RecA like protein antibody
    • RecA, E. coli, homolog of antibody
    • Recombination protein A antibody
    see all

Anti-Rad51 antibody 画像

  • 2BN hTert (XLF-deficient) human fibroblasts were fixed for 30 min with methanol at −20°C, dipped for 1 min in ice cold acetone for permeabilization and washed three times for 10 min with PBS/1% FCS. Non-specific antigens were blocked for 30 min in 5% BSA in PBS/1% FCS. Samples were incubated with ab63801 (1∶15000) in PBS/1% FCS over night at 4°C, washed three times in PBS/1% FCS and incubated for 1 h at room temperature with AlexaFluor 488 conjugated secondary antibodies (1 : 500). After three times of washing in PBS, cells were DAPI stained.

    Image shows 2BN hTert (XLF-deficient) human fibroblasts analyzed 2 h post IR with 1 Gy.

  • All lanes : Anti-Rad51 antibody (ab63801) at 1/2000 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : HeLa cell extract


    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • Immunofluorescent detection of Rad51 foci formation induced by DNA damage. Normal human diploid cells were irradiated by X-ray (0.5 Gly) and after incubation of 6 hr, the cells were fixed and immuno-stained by using ab63801 (x100 dilution) as the primary antibody and Alexa594 labeled anti-rabbit antibody as the secondary antibody.

Anti-Rad51 antibody (ab63801) 使用論文

This product has been referenced in:
  • Marin-Vicente C  et al. RRP6/EXOSC10 is required for the repair of DNA double-strand breaks by homologous recombination. J Cell Sci 128:1097-107 (2015). Read more (PubMed: 25632158) »
  • Danilowicz C  et al. The differential extension in dsDNA bound to Rad51 filaments may play important roles in homology recognition and strand exchange. Nucleic Acids Res 42:526-33 (2014). Read more (PubMed: 24084082) »

See all 18 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Dictyostelium discoideum Cell (AX3)
Permeabilization Yes - Triton X100
Specification AX3
Fixative Methanol
Username

Abcam user community

Verified customer

投稿 Feb 26 2016

These bands could be result of a few factors. First is that the antibody is detecting a second isoform of Rad51 with similar size which is present in the nucleus. but not in the cytoplasm. The SwissProt page for this target does list 4 isoforms (http:/...

Read More

Thank you for contacting us.

The following protocol was used with ab104306 for ICC/IF:

Preparation of slides:

1. Grow cultured cells on sterile glass cover slips at 37°C overnight.

2. Wash sample with PBS twice.<...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

I am happy to help. Best of luck in your experiments!

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab63801.

To check the status of the order please contact ou...

Read More

Thank you for your enquiry. Regarding question 1: No, these are two different rabbit anti-Rad51 antibodies. Regarding question 2: Both antibodies bind Rad51 and should label foci of cells induced with DNA damage. The ICC image on the datasheet of ab469...

Read More

Thank you for contacting us. We do not have small samples of any of our antibodies but ab63801 is covered by our Abpromise guarantee for ICC on human, mouse, or chicken samples. If you do not obtain the results you expect (e.g., staining of nuclear...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human mammary epithelial)
Specification human mammary epithelial
Fixative Paraformaldehyde
Permeabilization Yes - 1% triton in PBS
Blocking step 1% fish skin gelatin and 1% bovine serum albumin in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Activated B cells)
Specification Activated B cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton, 0.3%
Blocking step Serum as blocking agent for 45 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Oct 21 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"