製品の概要

  • 製品名Anti-Rad51 antibody
    Rad51 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rad51
  • アプリケーション適用あり: WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human Rad51 expressed in E. coli.

  • ポジティブ・コントロール
    • IF: HEK293 cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.035% Sodium Azide
    Constituents: 30% Glycerol, PBS, pH 7.5
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab46981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 37 kDa.
ICC/IF Use a concentration of 1 - 5 µg/ml. Acetone or methanol fixation is recommended.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
  • 組織特異性Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
  • 関連疾患Breast cancer
    Mirror movements 2
    Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
  • 配列類似性Belongs to the RecA family. RAD51 subfamily.
    Contains 1 HhH domain.
  • ドメインThe nuclear localization may reside in the C-terminus (between 259 and 339 AA).
  • 翻訳後修飾Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
    Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
  • 細胞内局在Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BRCA1/BRCA2 containing complex, subunit 5 antibody
    • BRCC 5 antibody
    • BRCC5 antibody
    • DNA repair protein RAD51 homolog 1 antibody
    • DNA repair protein rhp51 antibody
    • hRAD51 antibody
    • HsRAD51 antibody
    • HsT16930 antibody
    • MRMV2 antibody
    • Rad 51 antibody
    • RAD51 antibody
    • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
    • RAD51 homolog A antibody
    • RAD51 homolog antibody
    • RAD51 recombinase antibody
    • RAD51, S. cerevisiae, homolog of antibody
    • RAD51_HUMAN antibody
    • RAD51A antibody
    • RECA antibody
    • RecA like protein antibody
    • RecA, E. coli, homolog of antibody
    • Recombination protein A antibody
    see all

Anti-Rad51 antibody 画像

  • Staining of HEK293 cells with anti Rad51 antibody (ab46981, 1 µg/ml) in green, and DAPI in blue.
  • All lanes : Anti-Rad51 antibody (ab46981) at 0.5 µg/ml

    Lane 1 : Recombinant human Rad51 protein (ab63808) at 0.1 µg
    Lane 2 : Recombinant human Rad51 protein (ab63808) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

Anti-Rad51 antibody (ab46981) 使用論文

This product has been referenced in:
  • Wang S  et al. Arabidopsis BRCA2 and RAD51 proteins are specifically involved in defense gene transcription during plant immune responses. Proc Natl Acad Sci U S A 107:22716-21 (2010). WB ; Arabidopsis thaliana . Read more (PubMed: 21149701) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. Regarding question 1: No, these are two different rabbit anti-Rad51 antibodies. Regarding question 2: Both antibodies bind Rad51 and should label foci of cells induced with DNA damage. The ICC image on the datasheet of ab469...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"