Anti-Rad50 抗体 [EPR3466(2)] (ab124682)

製品の概要

  • 製品名Anti-Rad50 antibody [EPR3466(2)]
    Rad50 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3466(2)] to Rad50
  • アプリケーション適用あり: ICC/IF, WBmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide, corresponding to residues in Human Rad50.

  • ポジティブ・コントロール
    • K562, MOLT4, Jurkat and MCF7 cell lysates.
  • 特記事項

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 154 kDa).
  • 追加情報Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
    • 組織特異性Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
    • 関連疾患Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.
    • 配列類似性Belongs to the SMC family. RAD50 subfamily.
      Contains 1 zinc-hook domain.
    • ドメインThe zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus. Chromosome > telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DNA repair protein RAD50 antibody
      • hRAD50 antibody
      • hsRAD50 antibody
      • Mrell antibody
      • NBSLD antibody
      • Rad 50 antibody
      • RAD50 2 antibody
      • rad50 antibody
      • RAD50 homolog (S cerevisiae) antibody
      • RAD50 homolog antibody
      • RAD50 PEN antibody
      • RAD50, S. cerevisiae, homolog of antibody
      • RAD50_HUMAN antibody
      • RAD502 antibody
      • Rad50l antibody
      • Truncated RAD50 protein antibody
      see all

    Anti-Rad50 antibody [EPR3466(2)] 画像

    • ab124682 staining Rad50 in the U2OS cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 5% BSA for 60 minutes. Samples were incubated with undiluted primary antibody  for 2 hours. An undiluted FITC conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-Rad50 antibody [EPR3466(2)] (ab124682) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 154 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Anti-Rad50 antibody [EPR3466(2)] (ab124682) 使用論文

    ab124682 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Non-reduced Denaturing (8%)
    Sample Human Cell lysate - whole cell (MEFs, U2OS, HEK 293 and Fibroblasts)
    Specification MEFs, U2OS, HEK 293 and Fibroblasts
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Abcam user community

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    投稿 Nov 07 2014

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (U2OS)
    Specification U2OS
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Fixative Formaldehyde
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    投稿 Nov 03 2014

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (4-12%)
    Sample Human Cell lysate - whole cell (HeLa)
    Specification HeLa
    Treatment 10 Gy IR
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    投稿 Apr 25 2014

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (U2OS)
    Specification U2OS
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Apr 23 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Nov 19 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"