Anti-Rad22 抗体 (ab63800)
Key features and details
- Rabbit polyclonal to Rad22
- Suitable for: ELISA, WB, IP, ICC/IF
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
製品の概要
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製品名
Anti-Rad22 antibody -
製品の詳細
Rabbit polyclonal to Rad22 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, IP, ICC/IFmore details -
種交差性
交差種: Schizosaccharomyces pombe -
免疫原
Purified recombinant Rad22 protein (Schizosaccharomyces pombe)
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ポジティブ・コントロール
- Schizosaccharomyces pombe whole cell extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63800の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
1/2000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa). |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Rad22 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of S.cerevisiae and human Rad52 proteins, which play a major role in genetic recombination and recombination repair by mediating the strand annealing reaction between homologous DNA strands. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 2543086 Schizosaccharomyces pombe
- SwissProt: P36592 Schizosaccharomyces pombe
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別名
- DNA repair and recombination protein rad22 antibody
- DNA repair protein Rad22 antibody
- Rad 22 antibody
画像
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Anti-Rad22 antibody (ab63800) + S.pombe whole cell extract
Predicted band size: 52 kDa -
All lanes : Anti-Rad22 antibody (ab63800) at 1/2000 dilution (unpurified)
Lane 1 : Molecular weight markers
Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
Lane 3 : Whole cell extract of Schizosaccharomyces pombe rad22 deletion strain
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.ab63800 was used unpurified.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab63800 は 2 報の論文で使用されています。
- Hardy J et al. Histone deposition promotes recombination-dependent replication at arrested forks. PLoS Genet 15:e1008441 (2019). PubMed: 31584934
- Gómez-Escoda B & Wu PJ The organization of genome duplication is a critical determinant of the landscape of genome maintenance. Genome Res 28:1179-1192 (2018). PubMed: 29934426