Anti-RAD18 抗体 [EPR8942] (ab157463)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8942] to RAD18
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RAD18 antibody [EPR8942]
RAD18 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8942] to RAD18 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- 293T, HCT-116, K562 and HeLa cell lysates; Permeabilized K562 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR8942 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157463の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
|
特記事項 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 56 kDa. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the RAD18 family.
Contains 1 RING-type zinc finger.
Contains 1 SAP domain.
Contains 1 UBZ-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 56852 Human
- Entrez Gene: 58186 Mouse
- Entrez Gene: 362412 Rat
- Omim: 605256 Human
- SwissProt: Q9NS91 Human
- SwissProt: Q9QXK2 Mouse
- Unigene: 375684 Human
- Unigene: 103812 Mouse
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別名
- 2810024C04Rik antibody
- DNA repair protein rad18 antibody
- E3 ubiquitin-protein ligase RAD18 antibody
see all
画像
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All lanes : Anti-RAD18 antibody [EPR8942] (ab157463) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HCT-116 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa -
Intracellular flow cytometric analysis of permeabilized K562 cells labeling RAD18 with ab157463 at 1/10 dilution (red) compared to a rabbit IgG negative control antibody (green).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab157463 は論文での使用が確認できていません。