製品の概要
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製品名Anti-Rad17 (phospho S645) antibody [EP1519Y]
Rad17 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EP1519Y] to Rad17 (phospho S645)
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由来種Rabbit
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特異性ab75779 only detects Rad17 phosphorylated on Serine 645.
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アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC,IHC-P or IP -
種交差性交差種: Human
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免疫原
A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17
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ポジティブ･コントロール
- HeLa cell lysates either untreated or treated with Lambda Phosphatase.
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特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EP1519Y
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アイソタイプIgG
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研究分野
関連製品
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab75779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2500 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
ターゲット情報
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機能Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
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組織特異性Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
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配列類似性Belongs to the rad17/RAD24 family.
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翻訳後修飾Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
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細胞内局在Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
- Information by UniProt
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参照データベース
- Entrez Gene: 5884 Human
- Omim: 603139 Human
- SwissProt: O75943 Human
- Unigene: 16184 Human
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別名
- CCYC antibody
- Cell cycle checkpoint protein antibody
- Cell cycle checkpoint protein RAD17 antibody
see all
画像
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![Western blot - Anti-Rad17 (phospho S645) antibody [EP1519Y] (ab75779)](http://a.abcam.com/ps/datasheet/Images/75/ab75779/ab75779wb.gif)
Western blot - Anti-Rad17 (phospho S645) antibody [EP1519Y] (ab75779)All lanes : Anti-Rad17 (phospho S645) antibody [EP1519Y] (ab75779) at 1/5000 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates, treated
with Lambda
Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa
プロトコール
参考文献
ab75779 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"