Anti-Rad17 (phospho S645) 抗体 [EP1519Y] (ab75779)

製品の概要

• 製品名
  Anti-Rad17 (phospho S645) antibody [EP1519Y]
  Rad17 一次抗体 製品一覧
• 製品の詳細
  Rabbit monoclonal [EP1519Y] to Rad17 (phospho S645)
• 特異性
  ab75779 only detects Rad17 phosphorylated on Serine 645.
• アプリケーション
  適用あり: WBmore details
  適用なし: Flow Cyt,ICC,IHC-P or IP
• 種交差性
  交差種: Human
  
• 免疫原

  A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17

• ポジティブ･コントロール
  • HeLa cell lysates either untreated or treated with Lambda Phosphatase.
• 特記事項

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• バッファー
  PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• 精製度
  Tissue culture supernatant
• ポリ/モノ
  モノクローナル
• クローン名
  EP1519Y
• アイソタイプ
  IgG
• 研究分野
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • DNA Damage Recognition

関連製品

• Positive Controls
  • HeLa nuclear extract lysate (ab14655)

アプリケーション

ターゲット情報

• 機能
  Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
• 組織特異性
  Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
• 配列類似性
  Belongs to the rad17/RAD24 family.
• 翻訳後修飾
  Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
• 細胞内局在
  Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.

• Target information above from: UniProt accession O75943 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 5884 Human
  • Omim: 603139 Human
  • SwissProt: O75943 Human
  • Unigene: 16184 Human
• 別名
  • CCYC antibody
  • Cell cycle checkpoint protein antibody
  • Cell cycle checkpoint protein RAD17 antibody

  • FLJ41520 antibody
  • HRAD 17 antibody
  • hRad17 antibody
  • R24L antibody
  • Rad 17 antibody
  • Rad 24 antibody
  • RAD1 (S. pombe) homolog antibody
  • RAD1 homolog antibody
  • RAD17 antibody
  • RAD17 homolog (S. pombe) antibody
  • RAD17 homolog antibody
  • Rad17 like protein antibody
  • RAD17, S. pombe, homolog of antibody
  • RAD17_HUMAN antibody
  • RAD17Sp antibody
  • Rad24 antibody
  • Rad24, mouse, homolog of antibody
  • Rad24, S. cerevisiae, homolog of antibody
  • RF C activator 1 homolog antibody
  • RF C/activator 1 homolog antibody
  • RF-C/activator 1 homolog antibody

  see all

プロトコール

• Western blot protocols