Anti-Rad17 抗体 - Carboxyterminal end (ab50526)


  • 製品名Anti-Rad17 antibody - Carboxyterminal end
    Rad17 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rad17 - Carboxyterminal end
  • 特異性Ab50526 specifically recognizes RAD17 (C terminal). The immunizing peptide is present in isoforms 1 to 4 of RAD17.
  • アプリケーション適用あり: IP, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: HSAEESLGEPTQATVPET, corresponding to amino acids 623-640 of Human Rad17 (isoform 2) conjugated to KLH by an N terminal Cysteine residue linker.

  • ポジティブ・コントロール
    • HeLa cell lysates and HEK293-T cells fixed with methanol acetone.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Ab50526 is affinity purified on the immunizing peptide immobilized on agarose.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野



Our Abpromise guarantee covers the use of ab50526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
  • 追加情報ICC/IF: Use at a concentration of 5.0 - 10.0 µg/ml using HEK293-T cells fixed with methanol-acetone.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 77 kDa.
    Staining of the RAD17 band is specifically inhibited by the immunizing peptide. It is recommended that the antibody be diluted in phosphate buffered saline containing 5% non-fat dry milk and 0.05 % TWEEN 20.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
    • 組織特異性Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
    • 配列類似性Belongs to the rad17/RAD24 family.
    • 翻訳後修飾Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
    • 細胞内局在Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CCYC antibody
      • Cell cycle checkpoint protein antibody
      • Cell cycle checkpoint protein RAD17 antibody
      • FLJ41520 antibody
      • HRAD 17 antibody
      • hRad17 antibody
      • R24L antibody
      • Rad 17 antibody
      • Rad 24 antibody
      • RAD1 (S. pombe) homolog antibody
      • RAD1 homolog antibody
      • RAD17 antibody
      • RAD17 homolog (S. pombe) antibody
      • RAD17 homolog antibody
      • Rad17 like protein antibody
      • RAD17, S. pombe, homolog of antibody
      • RAD17_HUMAN antibody
      • RAD17Sp antibody
      • Rad24 antibody
      • Rad24, mouse, homolog of antibody
      • Rad24, S. cerevisiae, homolog of antibody
      • RF C activator 1 homolog antibody
      • RF C/activator 1 homolog antibody
      • RF-C/activator 1 homolog antibody
      see all

    Anti-Rad17 antibody - Carboxyterminal end 画像

    • Ab50526, at 10ug/ml, staining Rad17 in methanol-acetone fixed HEK-293-T cells by immunofluorescence.

    Anti-Rad17 antibody - Carboxyterminal end (ab50526) 使用論文

    ab50526 has not yet been referenced specifically in any publications.

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