The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500 - 1/15000. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
Belongs to the rad17/RAD24 family.
Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
ab2847 at a 1:10000 dilution staining ~90 kDa Rad 17 in HeLa whole cell lysate (30µg / lane, +/- 10 Gy IR, +/- calf intestinal phosphatase treatment (CIP)) by Western blot (ECL, 30 seconds). ab2847 at a 1:10000 dilution staining ~90 kDa Rad 17 in HeLa whole cell lysate (30µg / lane, +/- 10 Gy IR, +/- calf intestinal phosphatase treatment (CIP)) by Western blot (ECL, 30 seconds).