Anti-RACK1 抗体 [EPR7388] (ab129084)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7388] to RACK1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RACK1 antibody [EPR7388]
RACK1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7388] to RACK1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, HepG2, SH-SY5Y, MCF7, C6, PC-12, Mouse brain, and NIH/3T3 lysates. IHC-P: human colon carcinoma, and cervical carcinoma, mouse cerebrum, and rat cerebrum tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7388 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129084の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50 -1/100 |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 -1/100 |
ターゲット情報
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機能
Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation. -
配列類似性
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats. -
ドメイン
The WD repeats domain 5 mediates interaction with TRIM63. -
細胞内局在
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells. - Information by UniProt
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参照データベース
- Entrez Gene: 10399 Human
- Entrez Gene: 14694 Mouse
- Entrez Gene: 83427 Rat
- Omim: 176981 Human
- SwissProt: P63244 Human
- SwissProt: P68040 Mouse
- SwissProt: P63245 Rat
- Unigene: 5662 Human
see all -
別名
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2-rs1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling RACK1 with Purified ab129084 at 1:1000 dilution (0.201 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 6 : Mouse brain lysates
Lane 7 : Mouse spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
All lanes : Anti-RACK1 antibody [EPR7388] (ab129084) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : PC12 cell lysate
Lane 5 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa -
ab129084, at a dilution of 1/50, staining RACK1 in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab129084 は 6 報の論文で使用されています。
- Wu J et al. EST12 regulates Myc expression and enhances anti-mycobacterial inflammatory response via RACK1-JNK-AP1-Myc immune pathway. Front Immunol 13:943174 (2022). PubMed: 36003390
- Filho EGF et al. RACK1 plays a critical role in mast cell secretion and Ca2+ mobilization by modulating F-actin dynamics. J Cell Sci 134:N/A (2021). PubMed: 34550354
- Dan H et al. RACK1 promotes cancer progression by increasing the M2/M1 macrophage ratio via the NF-?B pathway in oral squamous cell carcinoma. Mol Oncol 14:795-807 (2020). PubMed: 31997535
- Li XY et al. RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer. Gastroenterol Res Pract 2019:5625026 (2019). PubMed: 30962803
- Kuppusamy M et al. Interaction of the Mineralocorticoid Receptor With RACK1 and Its Role in Aldosterone Signaling. Endocrinology 158:2367-2375 (2017). PubMed: 28472300
- Corcoran KA et al. Regulation of fear extinction versus other affective behaviors by discrete cortical scaffolding complexes associated with NR2B and PKA signaling. Transl Psychiatry 5:e657 (2015). PubMed: 26460481