製品の概要

  • 製品名Anti-RACGAP1 antibody
    RACGAP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RACGAP1
  • 特異性Detects total RACGAP1 levels
  • アプリケーション適用あり: IHC-P, WB, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic non-phosphopeptide (Human) from around the phosphorylation site of serine 387 (RISPGC)

  • ポジティブ・コントロール
    • COS7 or HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 20501863
WB 1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/40000.

ターゲット情報

  • 機能Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
  • 組織特異性Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase.
  • 配列類似性Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Rho-GAP domain.
  • ドメインThe coiled coil region is indispensible for localization to the midbody during cytokinesis.
  • 翻訳後修飾Phosphorylated at multiple sites in the midbody during cytokinesis. Phosphorylation by AURKB on Ser-387 at the midbody is, at least in part, responsible for exerting its latent GAP activity towards RhoA. Phosphorylation on multiple serine residues by PLK1 enhances its association with ECT2 and is critical for cleavage furrow formation.
  • 細胞内局在Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasmic vesicle > secretory vesicle > acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CYK4 antibody
    • GAP antibody
    • Gap1 antibody
    • GTPase activating protein antibody
    • HsCYK-4 antibody
    • ID GAP antibody
    • KIAA1478 antibody
    • Male germ cell RacGap antibody
    • MgcRacGAP antibody
    • Protein CYK4 homolg antibody
    • Protein CYK4 homolog antibody
    • Rac GTPase activating protein 1 antibody
    • Rac GTPase-activating protein 1 antibody
    • RACGAP 1 antibody
    • Racgap1 antibody
    • RGAP1_HUMAN antibody
    see all

Anti-RACGAP1 antibody 画像

  • All lanes : Anti-RACGAP1 antibody (ab61192) at 1/500 dilution

    Lane 1 : Cos7 cell extract
    Lane 2 : Cos7 cell extract with peptide


    Predicted band size : 71 kDa
    Observed band size : 71 kDa
  • Immunofluorescence analysis of HeLa cells labelled with ab61192 at 1/50 dilution. Samples were treated -/+ peptide (left and right images respectively).
  • IHC image of ab61192 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61192, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-RACGAP1 antibody (ab61192) 使用論文

This product has been referenced in:
  • Stone R  et al. Identification of genes correlated with early-stage bladder cancer progression. Cancer Prev Res (Phila Pa) 3:776-86 (2010). IHC-P ; Mouse . Read more (PubMed: 20501863) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
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投稿 Dec 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"